| 弹性蛋白酶和激肽释放酶的联产工艺 |
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| 引用本文： | 弹性蛋白酶和激肽释放酶的联产工艺[J]. 中国药科大学学报, 1988, (1): 25-29. |
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| 作者姓名： | 陈春麟 吴梧桐 魏天松 李继珩 |
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| 作者单位： | 中国药科大学生物化学教研室(陈春麟，吴梧桐)，山东省荷泽生化制药厂(魏天松)，中国药科大学生物化学教研室(李继珩) |
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| 摘 要： | 本文探讨了利用阴离子交换树脂直接从J_2树脂分离弹性蛋白酶后的母液中吸附胰激肽释放酶,然后经NH_4Ac洗涤和洗脱及丙酮沉淀而获得胰激肽释放酶的最佳工艺路线。每千克猪胰脏平均可得弹性蛋白酶3.09g(纯度为56u/mg),胰激肽释放酶2.72g(纯度为7.44u/mg).胰激肽释放酶回收率为56.6%。
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| 关 键 词： | 弹性蛋白酶 胰激肽释放酶 阴离子交换树脂 |
STUDY ON UNITED PRODUCTION OF ELASTASE AND KALLIKREIN |
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| STUDY ON UNITED PRODUCTION OF ELASTASE AND KALLIKREIN[J]. Journal of China Pharmaceutical University, 1988, (1): 25-29. |
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| Authors: | Chen Chunlin Wu Wutong Wei Tiansong Li Jihen |
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| Affiliation: | Chen Chunlin,Wu Wutong,Wei Tiansong~ and Li Jihen |
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| Abstract: | A new procedure for isolation of elastase and kallikrein was investigated. The procedure was that elastase was first adsorbed by J_2 resin, the kallikrein in the filtrate was then adsorbed by D resin, both elastase and kallikrein in he resin were eluted by NH_4Ac and precipitated by acetone. Average yields and activities of elastase and kallikrein were 3.09, 2.72g/kg pancreas and 56.00, 7.44u/mg, respectively. The recovery of Kallikrein was 56.6%. |
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| Keywords: | Elastase Kallikrein Resin |
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