大鼠创伤性脑损伤后Numbl的表达变化

目的：观察大鼠创伤性脑损伤后Numbl的表达变化及其与脑损伤的关系。方法：构建大鼠创伤性脑损伤模型,收集蛋白并做冰冻切片,采用Western Blot及免疫组化分析Numbl的表达变化及分布,免疫荧光双标分析Numbl的细胞定位。结果：大鼠创伤性脑损伤后Numbl的表达降低,其主要分布在损伤区域的皮质中,免疫荧光显示Numbl主要定位于皮质的神经元中。结论：Numbl在大鼠创伤性脑损伤后表达减少,其主要定位于损伤区域的神经元中,有可能参与损伤诱导的神经元的凋亡及再生。

The expression changes of Numbl in rat brain cortex after traumatic brain injury

Objective：To investgate the expresseion change of Numbl in the rat brain cortex after traumatic injury.Methods：Traumatic brain injury（TBI） model in adult rats were made and Western blot and immunohistochemistry analysis were used to investigate the dynamic changes of Numbl expression in the brain cortex.And then double immunofluorescence staining was used to investigate the localization of Numbl.Results：Numbl was present in normal brain.It decreased gradually,reached the lowest point at day 3 after TBI,and then increased during the following days.Double immunofluorescence staining showed that Numbl immunoreactivity（IR） was found in neurons,but not in astrocytes and microglia.Conclusion：Numbl may be involved in the pathophysiology of TBI and further research is needed to have a good understanding of its function and mechanism.