红芪多糖HPS-3体外诱导人胃癌MGC-803细胞凋亡的研究

目的:研究红芪多糖均一组分HPS-3体外对人胃腺癌MGC-803细胞增殖及凋亡的作用。方法:应用MTT法、显微荧光术和流式细胞术测定HPS-3对MGC-803细胞的增殖、细胞周期和诱导凋亡的影响,应用流式细胞术测定对Bc1-2和Bax蛋白表达的影响。结果:HPS-3对MGC-803细胞具有抑制增殖、G2/M期阻滞和诱导凋亡作用;HPS-3降低bcl-2蛋白表达,对Bax蛋白表达无明显作用。结论:HPS-3可通过抑制人胃腺癌MGC-803细胞的生长增殖、G2/M期阻滞、诱导细胞凋亡,降低bcl-2蛋白等作用机制发挥肿瘤作用。

Study of apoptosis induced by Hedysari Polysaccharide HPS-3 on human gastric cancer cell line MGC-803 in vitro

Object:To study the effect of Hedysari polysaccharide HPS-3 on the proliferation and apoptosis of human gastric cancer cell line MGC-803 in vitro.Methods:MTT assay,flow cytometry and fluorescence microscopy were used to examine the effects of HPS-3 in anti-proliferation,cell cycle arresting and apoptosis inductio n.Flow cytometry were used to detect the effects on protein expression of Bcl-2 and Bax.Results:HPS-3 could cause apparent inhibition on cell growth,induce G2/M phase arrest and apoptosis on MGC-803 cells.HPS-3 could down-regulate bcl-2 protein expression,but Bax protein expression showed unchanged.Conclusion:HPS-3 shows its anti-tumor effects by way of inhibiting the cell growth,arresting the G2/M phase,inducing apoptosis,down-regulating bcl-2 protein expression.