创伤性脑损伤后脑组织c-fos表达及细胞凋亡

目的研究创伤性脑损伤（TBI）后脑组织c-fos表达及神经细胞凋亡，探讨神经细胞凋亡在脑继发性损害中的作用，及c-fos基因表达与凋亡的关系。方法雄性Wistar大鼠，随机分为假手术对照组和脑创伤后3、12、24、72小时和7天组，采用自由落体撞击致重型颅脑损伤。应用TUNEL法检测神经细胞凋亡，免疫组化法观测c-fos的表达。结果TBI后3小时创伤组各组脑组织即出现c-fos表达和神经细胞凋亡，随伤后时间逐渐增强，至伤后72小时达高峰，伤后7天仍有明显的c-fos表达和神经细胞凋亡。其分布区域为脑挫伤区、挫伤周围皮质和海马，累及细胞类型包括神经元和胶质细胞。c-fos表达与神经细胞凋亡在时序空间分布上基本一致，两者呈正相关。结论创伤性脑损伤可引起脑组织明显的c-fos表达和神经细胞凋亡，c-fos表达增加在神经细胞凋亡和修复中可能起重要作用。

C-fos expression and apoptosis after traumatic brain injury

Objective To study c-fos expression and apoptosis after traumatic brain injury(TBI) in rats,and to explore the role of apoptosis in the secondary brain injury,and the relation between c-fos expression and neuroglial apoptosis.Methods Male Wistar rats were randomly divided into the control group and 3,12,24,72h and 7 day group after injury.Parietal brain contusion was made by weight-dropping method.Apoptosis was detected by immunohistochemical staining(TUNEL),and c-fos expression was detected by immunohistochemistry.Results c-fos expression and apoptosis was found in the injury group 3h after TBI,which increased gradually at 12,24,72h and 7d after TBI.The involved cells included neurons and glial cells.c-fos expression and neuroglial apoptosis appeared to be similar in temporal changes and spatial localization,with a high relation between these 2 variables.Conclusion It is highly suggested that TBI could induce significant c-fos expression and apoptosis after TBI,and c-fos expression might be highly responsible for the apoptosis,proliferation and renovation after TBI.