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| 硒化麒麟菜多糖抑制人喉癌细胞株Hep-2增殖的作用 | |
| 引用本文： | 白雪,林晨,江振友,袁桂秀,沈伟哉,李扬秋,唐渝.硒化麒麟菜多糖抑制人喉癌细胞株Hep-2增殖的作用[J].肿瘤防治研究,2007,34(4):249-252. |
| 作者姓名： | 白雪 林晨 江振友 袁桂秀 沈伟哉 李扬秋 唐渝 |
| 作者单位： | 1. 510632,广州,暨南大学医学院微生物学与免疫学教研室 2. 510632,广州,暨南大学医学院人体解剖学教研室 3. 510632,广州,暨南大学医学院血液病研究所 4. 510632,广州,暨南大学医学院生命科学院化学系 |
| 基金项目： | 广东省科研基金粤科技团队资助项目;广东省中医药管理局科研项目;广东省广州市科技局科技攻关项目 |
| 摘 要： | 目的 通过比较研究海藻麒麟菜多糖粗提物、无机硒化合物-亚硒酸钠（Na2SeO3）及其共价化合物-硒化麒麟菜多糖对人喉癌细胞株Hep-2的作用，探索新的具有抗肿瘤活性的有机硒化合物。方法 应用不同剂量的样品作用于Hep-2细胞株后，用MTT比色分析法计算肿瘤抑制率。结果 硒化多糖对Hep-2细胞的抑制率高于相同多糖浓度的麒麟菜多糖和相同硒浓度的亚硒酸钠（P〈0．05）。硒化多糖的抑制率可高达71．53％，其多糖IC50，与硒IC50，分别为1805μg／ml和7．0μmol／L，分别低于麒麟菜多糖的多糖IC50，（3034μg／m1）和亚硒酸钠的硒IC50（9．0μmol／L）。结论 硒化麒麟菜多糖具有抗Hep-2细胞增殖的活性，其抑制作用明显优于亚硒酸钠及天然麒麟菜多糖的抗肿瘤活性。 |
| 关 键 词： | 硒化麒麟菜多糖 亚硒酸钠 HEp-2 抗肿瘤 |
| 文章编号： | 1000-8578（2007）04-0249-04 |
| 收稿时间： | 2006-3-27 |
| 修稿时间： | 2006-03-272006-09-09 |
Inhibiting Effects of Eucheuma Polysaccharide Selenide on the Proliferation of Carcinoma Cell Line HEp-2 |
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| BAI Xue,LIN Chen,JIANG Zhen-you,YUAN Gui-xiu,SHEN Wei-zai,LI Yang-qiu,TANG Yu.Inhibiting Effects of Eucheuma Polysaccharide Selenide on the Proliferation of Carcinoma Cell Line HEp-2[J].Cancer Research on Prevention and Treatment,2007,34(4):249-252. | |
| Authors: | BAI Xue LIN Chen JIANG Zhen-you YUAN Gui-xiu SHEN Wei-zai LI Yang-qiu TANG Yu |
| Institution: | 1. Department of Microbioloy and Immunology , Ji′nan University , Guangzhou 510632 , China;2. Department of Anatomy , 3. Institute of Hematology , Medical College , 4. Department of Chemistry , Life Science College |
| Abstract: | Objective To investigate new antitumor selenium compound by studying the effect of Eucheuma Polysaccharide ( EP) , inorganic selenium compound--sodium selenite (Na2SeO3) and organoselenium compound-Eucheuma polysaccharide selenide (SeEP) on the proliferation of human larynx epidermoid carcinoma cell line Hep-2 in vitro. Methods HEp-2 cells were incubated with EP , Na2SeO3 and SeEP separately at different concentrations for 72 hours. The cell viability was measured by MTT colorimetric assay. Results The inhibitory ratios of SeEP are significantly higher than that of EP or Na2SeO3 at some polysaccharide and selemium concent rations ( P < 0. 05) ,and reached to 71. 53 % at 4000μg/ ml. The IC50s of SeEP are 1805μg/ ml by polysaccharide and 7. 0μmol/ L by selenide , which are lower than 3034μg / ml of EP and 9. 0μmol/ L of Na2SeO3respectively. Conclusion SeEP shows an antitumor effect on HEp-2 cells , and it is more potent than both EP and Na2SeO3 |
| Keywords: | HEp-2 |
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