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| TB-DNA PCR室内质控方法初探 | |
| 引用本文： | 丁飞,周静,武红权. TB-DNA PCR室内质控方法初探[J]. 贵州医药, 2002, 26(2): 118-119 |
| 作者姓名： | 丁飞 周静 武红权 |
| 作者单位： | 1. 遵义医院检验科,563001 2. 贵州省遵义市中心血站,563002 3. 贵州省人民医院细菌室,贵阳550002 |
| 摘 要： | 目的：探讨PCR检测TB－DNA的室内质控方法。方法：通过设立空白对照和BCG参照在3个实验室用3种试剂对TB－DNAPCR进行室内质控。结果：空白对照能很好地监测污染造成的假阳性，以不同衡释倍数的BCG不但能很好地检测试验的敏感度，且能对整个实验加以质控。结论：采用空白对照和BCG对TB－DNAPCR进行室内质控是一种理想的室内质控方法。 |
| 关 键 词： | 室内质控方法 聚合酶链反应 TB－DNA |
Quality control in Iaboratory methods of TB-DNA PCR |
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| Ding Fei,Zhou Jing,Wu Hong Quan. Quality control in Iaboratory methods of TB-DNA PCR[J]. Guizhou Medical Journal, 2002, 26(2): 118-119 | |
| Authors: | Ding Fei Zhou Jing Wu Hong Quan |
| Affiliation: | Ding Fei,Zhou Jing,Wu Hong Quan. Department of Test,Zunyi Hospital,Zunyi 563002 |
| Abstract: | Objective To study quality control in laboratory methods of TB-DNA PCR. Methods Using three reagents controls quality of TB-DNA PCR by blank control and Bacille calmette-Guenn (BCG) reference were carried in three laboratories. Results Blank control can monitor polluting positive reaction well and BCG of different rate of dilution not only detect sensitivity of reagents, but control quality of whole experiment. Conclusion Controling TB-DNA PCR by blank control and BCG reference is an idea method for quality control in laboratory. |
| Keywords: | PCR BCG Interior quality coutrol |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |