| 胶原-壳聚糖/bFGF培养人成纤维细胞生物特性研究 |
| |
| 引用本文： | 李沁华,钟喻.胶原-壳聚糖/bFGF培养人成纤维细胞生物特性研究[J].中国医学物理学杂志,2003,20(4):301-303. |
| |
| 作者姓名： | 李沁华 钟喻 |
| |
| 作者单位： | 1. 暨南大学,生物医学工程研究所,广东,广州,510632
2. 暨南大学,生物免疫研究中心,广东,广州,510632 |
| |
| 摘 要： | 目的：研究一种适于人成纤雏细胞生长的组织工程支架材料，确定碱性成纤维细胞生长因子(bFGF)的用量。材料与方法：采用胶原与壳聚糖整合bFGF做为人成纤维细胞生长的支架材料，用体外细胞培养法确定胶原与壳聚糖在材料中的配比，用体外细胞相容性直观法观察材料与人成纤维细胞的相容性。结果：选择出了胶原与壳聚糖在材料中较佳的配比，确定了bFGF在材料中的用量范围．人成纤维细胞在胶碌一壳聚糖／bFGF膜持续生长繁殖，具有生物降解性。材料与细胞呈低毒性反应。结论：胶原—壳聚糖／bFGF可做为组织工程的支架材料。
|
| 关 键 词： | 胶原—壳聚糖 碱性成纤维细胞生长因子（bFGF） 人成纤维细胞培养 细胞相容性 |
Biological feature of collagen-chitosan membrane with basicfibroblast growth factor for the culture of human fibroblast |
| |
| LI Qin-hua- ZHONG Yu.Biological feature of collagen-chitosan membrane with basicfibroblast growth factor for the culture of human fibroblast[J].Chinese Journal of Medical Physics,2003,20(4):301-303. |
| |
| Authors: | LI Qin-hua- ZHONG Yu |
| |
| Institution: | Institute of biomedical engineering Center for reproductive immunology research Jinan university- Guangzhou 510632- China |
| |
| Abstract: | The effect of collagen-chitosan membrane with different proportionate collagen and bFGF were investigated for culture human fibroblast. The optimum weight ratio of collagen/chitoson and bFGF were selected. Using culture human fibroblast technologies and cytotoxicity evaluated in vitro- Cell morphology was observed. Experimental results show that collagen-chitosan with bFGF promoted human fibroblast adhesion and supported cell proliferation for a long time. Furthermore collagen-chitosan membrane obviously degrade after 18 d when human fibroblast was exhibited fusion spreading- compacting and stabilize. Cytotoxic to human fibroblast was revealed very low. Collagen- chitosan with bFGF should be useful as a tissue engineering biomaterial scaffold for cell culture. |
| |
| Keywords: | bFGF collagen-chitosan |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
