胶质细胞生长因子、神经生长因子与泌乳素垂体瘤生长特性的相关性研究

目的探讨胶质细胞生长因子（GGF）、神经生长因子（NGF）与垂体腺瘤临床生物学特性的关系。对术前、术后生长作预测。方法免疫组织化学法分析GGF、NGF在86例泌乳素垂体瘤（PRL）中的表达，分析GGF、NGF的表达与PRL水平、垂体腺瘤微血管密度、侵袭性及卒中等的关系。用GGF、NGF分别干预不同阶段的PRL细胞，观察细胞生长、周期变化及血管生成情况。通过相关分析及卡方检验证明肿瘤生长速率与GGF、NGF有无相关性。结果GGF在卒中组、侵袭性垂体腺瘤、复发PRL中均强阳性表达（P〈0．05），微血管密度明显升高（P〈0．01）。NGF在卒中组、侵袭性垂体腺瘤、高泌乳素水平垂体腺瘤中的表达明显低于非卒中组、非侵袭性垂体腺瘤、低泌乳素水平的垂体腺瘤（P〈0．05）；在NGF作用下，微血管密度明显降低（P〈0．05）。GGF均与生长速率呈显著正相关，而NGF与PRL卒中、侵袭性呈负相关。结论GGF是促进PRL生长及侵袭性的重要因素之一，NGF能部分抑制PRL细胞的增殖。2者能间接反映PRL的增殖活性，为临床上评价PRL侵袭性、复发、治疗及预后提供重要的参考指标。

Relevance between GGF, NGF and growth characteristics of PRL

Objective To explore the relationship between glial growth factor (GGF), nerve growth factor (NGF) and growth characteristics of prolactinoma (PRL) and to evaluate pre and postoperative growth of PRL. Methods Immunohistochemistry was used to analyze expression of GGF and NGF in 86 cases of PRL and to analyze the relationship between expression of GGF, NGF and PRL level, invasion, stroke, microvessel density. Cells were cultured with GGF and NGF to observe cell growth, cell cycle and angiogenesis. The relationship between proliferation, growth rate and GGF, NGF was evaluated by rank correlation and Chi-square test. Results GGF expression was significantly higher in invasive, stroke and recurrent pituitary adenomas ( P ＜ 0.05 ).Microvessel density increased significantly ( P ＜ 0.01 ). NGF expression was significantly lower in invasive, apoplexy and recurrent adenomas ( P ＜ 0. 05 ). Microvessel density decreased dramatically with NGF interruption ( P ＜ 0.05 ). GGF showed a positive correlation with growth rate of PRL. NGF showed a negative correlation with invasion and stroke. Conclusions GGF is one of the factors facilitating growth and invasion of PRL while NGF can partly restrain proliferation of PRL cells. Expression of GGF and NGF indirectly reflects proliferation activity of PRL and can be used as markers to evaluate invasion, recurrence, treatment response and prognosis of PRL.