系统性红斑狼疮中Th17细胞相关细胞因子的变化

目的探讨系统性红斑狼疮（systemic lupus erythematosus,SLE）血浆中T辅助17（Th17）细胞相关的细胞因子白细胞介素（interleukin,IL）-17、IL-6、IL-23、IL-27和转化生长因子β（transforming growth factor-beta,TGF-β）水平的变化。方法 SLE组43例;健康对照组20例。采用酶联免疫吸附测定法测定血浆中IL-17、IL-6、IL-23、IL-27和TGF-β水平,分析它们的相关性。结果 SLE组与健康对照组比较,血浆中IL-17（74.32±22.76）ng/L vs（32.12±5.68）ng/L,IL-6（62.06±9.92）ng/L vs（37.98±15.41）ng/L,IL-23（30.29±12.59）ng/L vs（15.10±12.31）ng/L和IL-27（27.61±11.16）ng/L vs（16.17±8.35）ng/L的浓度都明显升高（P〈0.01）,TGF-β的血浆浓度则明显降低（35.80±11.65）ng/L vs（69.08±28.76）ng/L（P〈0.01）。IL-17与IL-6,IL-23和IL-27之间呈正相关（r=0.542、0.887、0.757,均P〈0.05）,与TGF-β之间则呈负相关（r=-0.268,P〈0.05）。结论 IL-17及其相关的细胞因子在SLE表达异常,IL-17在SLE发病中具有重要作用。

Change of Th17-related cytokine in systemic lupus erythematosus

Objective To discuss the levels of T helper（Th）17-related cytokines interleukin（IL）-17,IL-6,IL-23,IL-27 and transforming growth factor-beta（TGF-β） in plasma of systemic lupus erythematosus（SLE）.Methods The patients group included 43 patients of SLE with active renal disease and the control group included 20 healthy subjects.Plasma levels of IL-17,IL-6,IL-23,IL-27 and TGF-β were measured by enzyme-linked immunosorbent assay.And their correlations were analyzed.Results The levels of IL-17（74.32±22.76） ng/L vs（32.12±5.68） ng/L,IL-6（62.06±9.92） ng/L vs（37.98±15.41） ng/L,IL-23（30.29±12.59） ng/L vs（15.10±12.31） ng/L and IL-27（27.61±11.16） ng/L vs（16.17±8.35） ng/L were markly higher in SLE patients compared with healthy controls.The levels of TGF-β were significantly decreased in SLE patients than in healthy controls.Their difference was significant（35.80±11.65） ng/L vs（69.08±28.76） ng/L（P0.01）.IL-17 level was correlated positively with the levels of IL-6,IL-23 and IL-27（r=0.542,0.887,0.757,all P0.05）.IL-17 was correlated negatively with TGF-β（r=-0.268,P0.05）. Conclusion The expression of IL-17 and its related cytokines were abnormal in SLE,IL-17 played important role in the pathogenesis of SLE.