针刺影响下丘脑性肥胖大鼠脂代谢相关因素的分析

目的:通过针刺肥胖大鼠并作相关实验分析,探讨针刺减肥效应的某些机理。方法:将实验大鼠分为正常组、模型组、针刺组、西布曲明组,观察各组大鼠体重指数(Lee’s指数)、左肾周脂肪重量和脂肪细胞体积、脂质代谢、胰岛素、瘦素及神经肽Y的变化,分析其相互作用与联系。结果:针刺组与模型组相比体重指数、脂质代谢有明显改善(P<0.01),左肾周脂肪湿重降低,脂肪细胞体积减小,与西布曲明组比较针刺组作用更好(P<0.05);针刺组与模型组比较血清胰岛素、瘦素水平明显降低(P<0.01),西布曲明组与模型组比较血清胰岛素、瘦素水平也趋降低(P<0.05);针刺组与模型组比较瘦素与神经肽Y之间有协调趋向,但分布并不一致,西布曲明组则无明显变化。结论:针刺具有减小体重指数、改变脂肪细胞体积、调节脂代谢功能的作用,且可能通过协调机体胰岛素、瘦素及神经肽Y分泌水平而达到降脂减肥目的。

Analysis on the Relevant Factors Involving the Effect of Acupuncture in Improving Lipo-metabolism in Hypothalamic Obesity Rats

Objective: To study the mechanism of acupuncture in reducing body weight in hypothalamic obesity rats. Methods: 80 SD rats were randomly and evenly divided into normal control, model, acupuncture and medication groups. Obesity model was established by subcutaneous injection of 15% sodium glutamate solution (0.2 mL/10 g), once daily and continuously for 5 days. Lee’s index, body weight and length, serum total cholesterol (TCh), triglyceride (TG), high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) were detected with routine biochemical techniques, and serum insulin, leptin and neuropeptide Y contents assayed with radioimmunoassay. EA (dense-sparse waves, frequency of 100 Hz, a suitable strength inducing local muscular tremor) was applied to bilateral “Zusanli” (ST 36)-“Sanyinjiao” (SP 6), and “Guanyuan” (CV 4)-“Zhongwan”(CV 12) for 15 min, once daily and continuously for 28 days. Rats of medication group were fed with Sibutramine (4 mg/kg/d, 28 days altogether). Results: Compared with normal control group, Lee’s index, wet weight of the left perirenal adipose tissue, the area of the lipocytes, serum TG, TCh and LDL-Ch, insulin, leptin and NPY contents and NYP content of the hypothalamus tissue of model group increased significantly (P<0.01); whereas, lipocyte number, serum HDL-Ch and lipoprotein lipase (LPL) and hypothalamic leptin contents of model group decreased significantly (P< 0.01). In comparison with model group, body weight, Lee’s index, wet weight of the left perirenal adipose tissue, area of the lipocytes, serum TG, TCh and LDL-Ch, insulin, leptin and NPY and hypothalamic NPY contents of acupuncture and medication groups lowered considerably (P<0.05～0.01), while the number of lipocytes, serum HDL-Ch and lipoprotein lipase (LPL) and hypothalamic leptin contents of acupuncture and medication groups increased significantly (P<0.05～ 0.01). Comparison between acupuncture and medication groups showed that Lee’s index, serum TG, insulin, leptin and NPY and hypothalamic NPY contents of acupuncture group were significantly lower than those of medication group (P<0.05～0.01), while the number of lipocytes, serum LPL and hypothalamic leptin contents of the former group were significantly higher than those of the later group (P<0.05～0.01). Conclusion: Acupuncture has a good effect in reducing body weight by improving lipoprotein metabolism and regulating blood insulin, blood and hypothalamic leptin and NPY secretion.