|
|||||
|
|
| 高效液相色谱法测定丹墨胶囊中丹酚酸B含量 | |
| 引用本文： | 林生文,李瑞明,陈孝,隆颖,曹媛,吴敏江,任斌.高效液相色谱法测定丹墨胶囊中丹酚酸B含量[J].中国药业,2011,20(21):20-21. |
| 作者姓名： | 林生文 李瑞明 陈孝 隆颖 曹媛 吴敏江 任斌 |
| 作者单位： | 1. 广东省药品检验所,广东广州,510180;中山大学附属第一医院,广东广州,510080 2. 中山大学附属第一医院,广东广州,510080 3. 广东省药品检验所,广东广州,510180 |
| 基金项目： | 广州市重大科技专项计划项目，广东省中医药局课题 |
| 摘 要： | 目的建立测定色谱丹墨胶囊中丹酚酸B含量的高效液相色谱法。方法色谱柱为Ecosil C18柱(200 mm×4.6 mm,5μm),流动相为乙腈-甲醇-甲酸-水(10∶30∶1∶59),检测波长286 nm。结果丹酚酸B进样量在0.120 042～2.400 84μg范围内与峰面积呈良好的线性关系,平均回收率为103.40%,RSD为0.70%(n=6)。结论该方法简便、灵敏、准确,可用于丹墨胶囊的质量控制。 |
| 关 键 词： | 高效液相色谱法 丹墨胶囊 丹酚酸B 含量测定 |
Determination of Salvianolic Acid B in Danmo Capsules by HPLC |
|
| Lin Shengwen,Li Ruiming,Chen Xiao,Long Ying,Cao Yuan,Wu Minjiang,Ren Bin.Determination of Salvianolic Acid B in Danmo Capsules by HPLC[J].China Pharmaceuticals,2011,20(21):20-21. | |
| Authors: | Lin Shengwen Li Ruiming Chen Xiao Long Ying Cao Yuan Wu Minjiang Ren Bin |
| Institution: | Lin Shengwen1,2,Li Ruiming2,Chen Xiao2,Long Ying1,Cao Yuan2,Wu Minjiang2,Ren Bin2(1.Guangdong Institute for Drug Control,Guangzhou,Guangdong,China 510180,2.Department of Pharmacy,the First Affiliated Hospital of Sun Yatsen University,China 510080) |
| Abstract: | Objective To establish a method for the content determination of salvianolic acid B in Danmo Capsules by HPLC.Methods Column was Ecosil C18（200 mm×4.6 mm,5 μm）;mobile phase was acetonitrile-methanol-formic acid-water（10 ∶30 ∶1 ∶59）,UV detection wavelength was at 286 nm.Results There was a good linear relationship of salvianolic acid B in the range of 0.120 042-2.400 84 μg.The average recovery rate was 103.40%,RSD was 0.70%（n=6）.Conclusion This method is simple,accurate and reliable.It can be used for quality control of Danmo Capsules. |
| Keywords: | HPLC Danmo Capsules salvianolic acid B content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |