| 高效液相色谱法测定土霉素片的含量 |
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| 引用本文： | 刘东育,李岩. 高效液相色谱法测定土霉素片的含量[J]. 中国药业, 2011, 20(24): 36-37 |
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| 作者姓名： | 刘东育 李岩 |
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| 作者单位： | 1. 吉林省辽源市食品药品检验所,吉林,辽源,136200
2. 吉林省白城市食品药品检验所,吉林,白城,137000 |
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| 摘 要： | 目的对土霉素片进行质量控制。方法采用高效液相色谱法测定土霉素片的含量。色谱柱为Agilent C18柱(250 mm×4.6 mm,5μm),流动相为0.1%磷酸-甲醇(42∶58),检测波长为254 nm,柱温为35℃。结果土霉素进样量在1.6～2.4μg范围内与峰面积呈良好线性关系,平均回收率为99.88%,RSD=0.31%(n=6)。结论该方法简便易行、重现性好,可用于土霉素片的质量控制。
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| 关 键 词： | 高效液相色谱法 土霉素 含量测定 |
Content Detemunation of Oxytetracycline by HPLC |
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| Liu Dongyu,Li Yan. Content Detemunation of Oxytetracycline by HPLC[J]. China Pharmaceuticals, 2011, 20(24): 36-37 |
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| Authors: | Liu Dongyu Li Yan |
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| Affiliation: | Liu Dongyu1,Li Yan2(1.Liaoyuan Institute for Food and Drug Control,Liaoyuan,Jilin,China 136200,2.Baicheng Institute for Food and Drug Control,Baicheng,China 137000) |
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| Abstract: | Objective To control the quality of oxytetracycline. Methods The content of oxytetracycline was determined by HPLC method. The chromatographic column was Agilent C18 column （250mm×4.6mm,5μm） with the mobile phase of 0.1% phosphoric acid-methanol （42 ： 58）, the detection wavelength at 254 nm and the column temperature was 35 ℃. Results The sample size of oxytetracycline in the range of 1.6- 2.4 μg showed the good linear relation with the peak areas. The average recovery rate was 99.88%, RSD =0.31% （n =6）. Conclusion This method is simple, easy and convenient with good reproducibility, which can be used to control the quality of oxytetracycline. |
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| Keywords: | HPLC oxytetracycline content determination |
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