白花曼陀罗悬浮培养细胞生长和生物碱活性成分合成

目的 研究白花曼陀罗细胞悬浮培养合成生物碱。方法 在MS液体培养基上悬浮培养细胞生产生物碱，以碳源、pH值、接种量等为影响因子进行研究。结果 蔗糖为最佳碳源，细胞接种量以鲜重1.5～2.0g／L、培养液初始pH值5．5～6．0对曼陀罗悬浮培养细胞生长和生物碱合成最有利，加入苯丙氨酸能促进生物碱的合成。结论 白花曼陀罗细胞悬浮培养能有效地合成生物碱。

Cell growth and alkaloid synthesis in suspension cultures of Datura metel

Object To investigate the synthesis of alkaloids by cell suspension cultures of Datura metel L. Methods Cultured cells derived from leaves of D. metel were m aintained in Murashige and Skoog (MS) liquid medium. The effects of different c arbohydrated, different inoculum sizes, and different pH values of medium as aff ecting facters on cell growth and alkaloid formation were studied. Re sults The optimum carbohydrate was sucrose, inoculum size (1.5 - 2. 0 g/L) for fresh weight and initial pH values (5.5-6.0) of the medium were benefit for cell growth and alkaloid synthesis in the suspension cultures. The synthesis of alk aloids was improved when exogenous phenylalanine added to the culture. Conclusion Suspension-cultured cells of D. metel c an be used to synthesize alkaloids.