基质金属蛋白酶及其组织抑制物在卵巢肿瘤中的表达

背景与目的：有研究表明,基质金属蛋白酶（metalloproteinases,MMPs）与肿瘤的发生发展,尤其是肿瘤细胞的侵袭和转移密切相关。本研究探讨基质金属蛋白酶MMP－2、MMP－9及其组织抑制物（tissue inbibitor of metalloproteinases-2,TIMP-2）与卵巢癌侵袭及卵巢癌生物学行为的关系。方法：采用免疫组化S－P法对128例卵巢肿瘤组织MMP－2、MMP－9及TIMP－2蛋白表达进行检测,并用Χ^2检验进行统计学分析。结果：免疫组化结果表明,MMP－2、MMP－9及TIMP－2在卵巢癌组织中阳性表达率明显高于卵巢囊腺瘤（P＜0．01）。MMP－2及MMP－9在卵巢癌临床分期Ⅲ-Ⅳ期病例中的阳性表达率明显高于I－Ⅱ期者,病理学分级3级病例中的表达率明显高于1－2级者（P＜0．01）,有淋巴结转移病例中的表达率明显高于无淋巴结转移者（P＜0．01）。而TIMP－2的阳性表达与MMP－2及MMP－9相反。结论：MMP－2、MMP－9及TIMP－2的表达与卵巢癌的临床分期、病理分级及淋巴结转移有关,MMP－2及MMP－9蛋白的高表达可作为判断卵巢部具有转移倾向的临床参考指标。

Expression of matrix metaloproteinase and tissue inhibitors in ovarian tumors

BACKGROUND & OBJECTIVE: Metalloproteinase(MMPs) is considered to be associated with the development of carcinoma, especially with the invasion and metastases of cancer. This study was designed to evaluate the relationship between the expression of MMP-2, MMP-9, and TIMP-2 and biological behavior of ovarian cancer. METHODS: The expression of MMP-2, MMP-9, and TIMP-2 in 128 patients with ovarian tumor was determined using immunohistochemical methods, and their relationship to many clinicopathological parameters was analyzed. RESULTS: Immunohistochemical studies revealed a diffuse granular distribution of MMP-2, MMP-9, and TIMP-2 in the cytoplasm of the carcinoma cells. The expression of MMP-2, MMP-9, and TIMP-2 was stronger in ovarian adenocarcinoma tissue than in ovarian benign tumor tissue (P < 0.01). MMP-2 and MMP-9 levels were found to be significantly higher in grade III-IV adenocarcinoma than in grade I-II adenocarcinoma (P < 0.01). MMP-2 and MMP-9 levels were significantly higher in G3 than in G1-2 tumors. Furthermore, the expression rate of MMP-2 and MMP-9 was higher in cases with lymph node metastases thanin those with non-lymph node metastasis. TIMP-2 expression was contrary to the expression of MMP-2 and MMP-9 on that three respects in ovarian carcinomas. CONCLUSION: The expression of MMP-2, MMP-9, and TIMP-2 correlate with tumor stage, poor differentiation, and lymphonodes metastases, which suggests that co-expression of MMP-2, MMP-9, and TIMP-2 within the same tumor seem to play an important role in the progression of ovarian cancer and could be useful prognostic markers for patients with ovarian carcinoma.