补骨脂中苯并呋喃苷类成分在大鼠血浆中的移行特征

目的:考察补骨脂中苯并呋喃苷类化合物(补骨脂苷、异补骨脂苷)在大鼠血浆的移行特征。方法:大鼠灌胃给予苯并呋喃苷部位提取物,采用HPLC-UV测定大鼠血浆中补骨脂苷、异补骨脂苷的含量,流动相0.1%甲酸水溶液-乙腈梯度洗脱,检测波长246 nm,血药浓度-时间数据采运用SPSS 11.0软件分析。结果:补骨脂苷和异补骨脂苷在15 min后在血液中可检测到,于1.5 h均达峰浓度,苯并呋喃苷类成分在大鼠体内吸收较快,补骨脂苷、异补骨脂苷在大鼠体内能转化为补骨脂素、异补骨脂素。结论:苯并呋喃苷类成分血浆HPLC特征性及专属性好,适用于该部位血浆移行成分的分析,为补骨脂相关制剂的临床前药动学研究提供参考。

Transitional Features of Benzofuran Glycosides Part from Psoraleae Fructus in Rat Plasma

Objective: To invesitgate transitional features of rat plasma after administration of benzofuran glycosides part from Psoraleae Fructus. Method: A HPLC-UV method was established to determine contents of psoralenoside and isopsoralenoside in rat plasma after administration of benzofuran glycosides part,mobile phase composed of acetonitrile and 0.1% formic acid water in gradient elution,detection wavelength was set at 246 nm.Plasma concentration-time data were dealed with SPSS 11.0 software. Result: Psoralenoside and isopsoralenoside appeared in plasma after 15 min of administration of benzofuran glycosides with 0.003 g·g^-1,and reached the peak concentration in 1.5 h,also their metabolites (psoralen and isopsoralen) were found in plasma. Conclusion: Specificity and characteristic of plasma HPLC are good,which is successfully used for quantification of psoralenoside and isopsoralenoside in rat plasma after administration of benzofuran glycosides part,benzofuran glycosides can be transformed into corresponding coumarins in rat.