大豆甙元(S86019)与乳香有效成分Bc-4或阿糖胞苷对HL-60细胞分化的联合诱导

大豆甙元($86019)单独处理HL-60细胞,对细胞生长诱导分化作用较弱,NBT阳性细胞数低于10%。当S86019与乳香有效成分Bc-4联合应用时,对HL-60细胞的生长有明显抑制,并有明显的分化诱导;S86019与Bc-4或Ara-c联合应用,对细胞的增殖有较强的抑制和分化诱导作用。S86019与Bc-4联合应用4d,NBT阳性细胞达80%,75%细胞获得吞噬乳胶颗粒能力,90%细胞向成熟粒细胞方向分化;S86019与Ara-c联合应用4d,NBT阳性反应细胞达70%,82%细胞获得吞噬能力,90%细胞形态发生分化。S86019与Bc-4或Ara-c联合用药4d,可明显阻断细胞由G1期向s期移行,G1期细胞数明显增加。

COMBINATION INDUCTION OF CELL DIFFERENTIATION OF HL-60 CELLS BY DAIDZEIN (S86019) AND BC-4 OR ARA-C

Experiments demonstrated that the cell differentiation of HL-60 cells induced by low concentrations of daidzein (S86019), BC-4 (active principle of Boswellia carterii Birdw) and Ara-C was not impressive. However, when they were used in combination 80% of HL-60 cells exhibited NBT reduction and 82% of the cells showed phagocytosis after four days exposure to daidzein and BC-4. When HL-60 cells were exposed to daidzein and Ara-c, 70% of the cells exhibited NBT reduction and phagocytosis. Flow cytometry indicated that the majority of the cells were blocked at G1 phase under the induction of combination of daidzein with BC-4 or Ara-C.