肾综合征出血热IgM抗体早期诊断两步法MacELISA的建立

目的 建立和改善肾综合征出血热早期诊断IgM抗体捕获MacELISA法.方法 汉坦病毒核蛋白重组表达纯化后用辣根过氧化物酶标记,建立一种以酶标抗原为基础的MacELISA(称为两步法MacELISA)并与常规三步法MacELISA进行比较分析.结果 检测不同病日HFRS患者血清IgM抗体比较两步法与三步法MacELISA高度相关,敏感性和特异性为100%,无有明显差别.结论 本方法操作简单,用时少,成本低,适合用于肾综合征出血热早期诊断和汉坦病毒感染的监测.

Development and application of a two-step MacELISA for the early diagnosis of hemorrhagic fever with renal syndrome

OBJECTIVE: To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure. METHODS: The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA. RESULTS: This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%. CONCLUSION: This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.