羊膜移植对碱烧伤大鼠角膜缘干细胞的影响

目的观察羊膜移植对碱烧伤大鼠角膜缘干细胞的影响。方法SD大鼠40只制作眼碱烧伤模型，单眼行眼碱烧伤；随机选取20只大鼠（20眼）为实验组，20只大鼠（20眼）为对照组；实验组行新鲜羊膜移植，对照组不处理，观察大鼠眼表情况。并于术后1周、2周、3周、4周取角膜缘组织，采用免疫组化技术观察p63蛋白在角膜缘干细胞的表达情况。结果p63在实验组和对照组的角膜缘干细胞中均有表达，位于角膜缘上皮细胞基底层的细胞核内。但实验组明显高于对照组，两者比较有统计学意义（P〈0．05）。且随着烧伤时间的延长，p63的表达呈线性下降趋势。结论眼碱烧伤后行羊膜移植，能促进角膜缘干细胞的增生、分化，利于角膜上皮的修复。

The influence of amniotic membrane transplantation to corneal limbal stem cells of alkali burned rats

Objective To observe the influence of amniotic membrane(AM) transplantation to corneal limbal stem cells of alkali burned rats. Methods Corneal alkali burned animal models were established with 40 SD rats and only one eye was involved. The animal models were divided randomly to two groups, the experimental group and control group, with 20 eyes in each group. The experimental group received frash AM transplantation. No treatment was applied in the control group. We observe the ocular manifestations. The corneal limbal tissues were drawn at 1 week, 2 weeks ,3 weeks and 4 weeks postoperatively.The expression of P63 protein around the corneal limbus was detected by immunohistochemistry. Results P63 expressed in corneal limbal stem cells in both experimental group and control group which located in the cellular nucleus of limbal basal epithelia. The expression of P63 was higher in the experimental group than the control group. There was statistically significant differences between them (P ＜ 0. 05 ). The expression of P63 had a tendency to decline linearly with the prolongation of burning time. Conclusion AM transplantation after alkali injury can promote the proliferation and differentiation of corneal limbal stem cells and help the recovery of corneal epithelia.