大肠癌组织PTEN和Skp2蛋白表达与预后相关性的探讨

目的:观察大肠癌组织中PTEN缺失和Skp2表达情况,并观察两者与大肠癌临床病理特征和预后的关系,探讨其在大肠癌预后的价值.方法:采用SP方法检测大肠癌组(61例)和对照组(20例)中PTEN和Skp2蛋白的表达,分析其表达与患者临床病理特征和5年生存率的关系,并观察两者之间的相关性.结果:大肠癌组PTEN表达率(72.13％)明显低于对照组(100.00％)；大肠癌组Skp2表达率(52.46％)明显高于对照组；PTEN缺失和Skp2的表达均与组织分化程度、淋巴结转移和临床分期呈正相关.PTEN阴性组的5年生存率(31.86％)低于阳性组(83.97％),Skp2阳性组的5年生存率(58.33％)低于阴性组(82.76％).PTEN和Skp2的表达呈负相关,r=-0.445,P=0.001.结论:PTEN缺失和Skp2表达可能与大肠癌发生、转移和预后有关,联合检测PTEN和Skp2蛋白可作为预测大肠癌发生转移和预后的指标.

Prognostic value of PTEN and Skp2 expression in colorectal carcinoma tissues

OBJECTIVE: To investigate expression of PTEN and of Skp2 in colorectal cancer and aseess their correlation to clinical pathological diameters and prognosis.METHODS:Enhanced immunohistochemical staining(SP) was applied to detect expression of PTEN and of Skp2 in colorectal cancer group and control group.Correlations of expression of PTEN and Skp2 to clinical pathological parameters and prognosis and their correlations were analysed.RESULTS: The positive rate of PTEN in colorectal cancer group(72.13%) were lower than those in control group(100%).The positive level of Skp2 in colorectal group(52.46%) were higher than those in control group.Loss of expression of PTEN and expression of Skp2 were positively correlated with the degree of differentitation,lymphatic metastasis,clinical stage.Moreover,the 5-year survival rates were lower in patients with PTEN negative expression(31.86%) than those with positive expression(83.97%).The 5-year survival rates were lower in patients with positive Skp2 expression(58.33%) than those negative expression(82.76%).The correlation of PTEN with Skp2 was negative(r=-0.445,P=0.001).CONCLUSIONS: Loss of expression of PTEN and expression of Skp2 may be closely related to the carcinogenesis,metastasis and prognosis of colorectal cancer.Joint detection of PTEN and Skp2 may be a good prognostic factor for carcinogenesis,metastasis and pronosis in colorectal cancer.