rs6983267和rs1447295与北京地区前列腺癌的关联研究

目的:探讨染色体8q24两个单核苷酸多态性位点(SNPs) rs6983267和rs1447295,与北京地区前列腺癌(PCa)的相关性.方法:采用病例-对照研究,入选了154例PCa患者和138名正常对照者,检测2个SNPs在病例组和对照组间的等位基因及基因型的分布情况,并分析危险基因型的累积效应.结果:rs6983267有TT、GT及GG 3种基因型,在病例组的分布分别为33.1％、46.4％及20.5％,在对照组的分布分别为32.6％、48.5％及18.9％;rs1447295也有AA、AC及CC 3种基因型,在病例组的分布分别为3.3％、38.2％及58.6％,在对照组的分布分别为1.5％、32.1％及66.4％.2个SNPs的风险等位基因和基因型频率在病例组和对照组间的分布差异无统计学意义,P＞0.05.与不具有风险基因型的个体相比,具有1个和同时具有2个风险基因型的个体PCa的发病风险虽然分别提高了1.231和1.571倍,但差异无统计学意义,P＞0.05.结论:SNPs rs6983267和rs1447295可能与北京地区PCa的易感性无关.

Association between rs6983267 and rs1447295 and the risk of prostate cancer in Beijing Han population

OBJECTIVE:To investigate the association of the two common single nucleotide polymorphisms(SNPs,rs6983267 and rs1447295) on chromosome 8q24 with the susceptibility of prostate cancer(PCa) in Beijing Han population.METHODS: Using a case-control design,154 PCa patients and 138 cancer-free healthy subjects were included.The distribution of allele and genotype frequencies of the SNPs rs6983267 and rs1447295 was detected with the polymerase chain reaction-high resolution melting curve(PCR-HRM) combined with gene sequencing,and analyzed the cumulative effect of risk genotypes of these two SNPs.RESULTS: AS for the genotypes of rs6983267,the distribution in the PCa group was as follow:33.1% TT,46.4% GT,20.5% GG,as compared with 32.6%,48.5% and 18.9% in the healthy controls.As regards the genotypes of rs1447295,there were 3.3% AA、38.2% AC and 58.6% CC in the PCa patients,in comparison with 1.5%、32.1% and 66.4% in the controls.No significant difference was observed between these two SNPs and the risk of PCa(P>0.05).And although results were not any statistically significant,men who carried one or two risk genotypes had a increased risk by 1.231 times and 1.571 times respectively,compared with men without any risk genotypes(P>0.05).CONCLUSION: SNPs rs6983267 and rs1447295 on chromosome 8q24 might not be correlated with PCa in Beijing Han population.