VEGF基因-460T/C单核苷酸多态性与河北地区汉族人群肺癌发病风险相关性分析

目的:研究血管内皮生长因子(VEGF)基因启动子区-460T/C单核苷酸多态性(SNP)与河北地区汉族人群肺癌发病风险的关系。方法:采用基于医院的病例-对照研究方法,采集200例肺癌患者和204名健康对照的静脉血,同时记录其病史和个人相关资料。以蛋白酶K消化-饱和氯化钠盐析法提取外周血白细胞DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法和引物介导的限制性聚合酶链反应(PIRA-PCR)方法检测VEGF-460T/C多态性位点的基因型。结果:肺癌组VEGF-460C/T SNP C等位基因频率(27.5%)明显高于对照组(20.1%),两组比较差异有统计学意义,χ2=6.109,P=0.013。肺癌组与对照组的T/T、T/C+C/C基因型频率分别为52.2%、47.5%和63.2%、36.7%,差异有统计学意义,χ2=4.445,P=0.029。与T/T基因型相比,携带C等位基因(T/C+C/C)的基因型可显著增加肺癌的发病风险;与T/T基因型相比,携带C等位基因(T/C+C/C)的基因型可显著增加不吸烟人群的发病风险。与T/T基因型相比,携带C等位基因的基因型(T/C+C/C),明显增加肺鳞状细胞癌及小细胞癌发病风险。结论:VEGF-460T/C SNP可能与肺癌发病风险相关。

Relationship between single nucleotide polymorphisms of -460T/C in VEGF gene and the susceptibilitiy of lung cancer in Hebei province

OBJECTIVE: To investigate the relationship between single nucleotide polymorphisms of -460T/C in the promoter region of VEGF gene and the susceptibilitiy of lung cancer in Hebei province.METHODS: This hospital-based case-control study included 200 lung cancer patients and 204 healthy controls.Genomic DNA was extracted by using proteinase K digestion followed by a salting out procedure.Polymorphisms of VEGF gene were analyzed by PCR-restriction fragment length polymorphism analysis(PCR-RFLP) and primer-introduced restriction analysis PCR(PIRA-PCR).RESULTS: The frequency of the VEGF-460 C/T SNP C allele in lung cancer patients(27.5%) was significantly higher than that in controls(20.1%,χ2=6.109,P=0.013).The frequencies of three genotypes(T/T and C/T+C/C) in controls were 63.2% and 36.7% respectively,and in lung cancer patients were 52.2% and 47.5% respectively.There was significant difference between the two groups(χ2=4.445,P=0.029).Compared with individuals with the T/T genotype,individuals with the T/C+C/C genotype had significantly higher risk to develop lung cancer.Compared with individuals with the T/T genotype,the T/C+C/C genotype increased the risk in developing lung cancer in non-smokers.When stratified by tumor histology,compared with individuals with the T/T genotype,individuals with the T/C+C/C genotype carried an increased risk of lung cancer in squamous cell carcinomas and small cell lung carcinomas.CONCLUSION: VEGF -460T/C SNP is associated with the risk of lung cancer.