喉癌p16区域的杂合性丢失和微卫星序列不稳定性分析

目的 缩小喉癌抑癌基因的寻找范围，探讨Ｐ１６基因在喉癌发生中的作用。方法 选择Ｐ１６基因附近５个微卫星多态标记对６０例喉癌进行杂合性丢失和微卫星序列不稳定性分析。结果 ５个标记在喉癌中杂合性丢失频率均不高，最高仅达２３．１％，但２个标记的微卫星序列不稳定性的频率较高，其中示记微卫星序列恶性循环频率高达４６．１％。结论 提示Ｐ１６基因在喉癌的发生中不以缺失为主，在Ｄ９Ｓ１７５２附近可能存在参与喉癌演

Loss of heterozygosity and microsatellite instability in laryngeal carcinoma near p16 gene

Objectvie To search for the minimal overlap region of tumor suppressor gene in laryngeal carcinoma and discuss the correlation of p16 gene with laryngeal carcinogenesis.Methods Five microsatellite polymorphism markers near p16 gene were selected to detect loss of heterozygosity (LOH) and microsatellite instability (MI) in 60 cases of laryngeal squamous cell carcinoma.Results The frequencies of LOH in 5 markers were less than 23.1%, while the frequencies of MI in 2 markers were higher, with the highest frequency (46.1%) in D9S1752. Conclusion The results suggest that the deletion of p16 gene does not play an important role in the laryngeal carcinogenesis and there may exist a gene around D9S1752 participating in the development of laryngeal carcinoma, which correlates with the mutation of repair gene.