| 复合PCR-RFLP技术检测ABO基因型 |
| |
| 引用本文： | 杨庆恩,朱传红.复合PCR-RFLP技术检测ABO基因型[J].中华医学遗传学杂志,1999,16(2):110-112. |
| |
| 作者姓名： | 杨庆恩 朱传红 |
| |
| 作者单位： | 1. 430030,武汉,同济医科大学法医学系
2. 武汉市刑事科学技术研究所 |
| |
| 摘 要： | 目的研究复合聚合酶链反应－限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）技术分型人类ＡＢＯ基因型。方法在每一个反应管内同时扩增ＡＢＯ糖基转移酶基因中２个特异性片段，然后于同一管内进行ＫｐｎⅠ和ＡｌｕⅠ限制酶消化，经聚丙烯酰胺凝胶电泳及银染，对武汉地区１２５例汉族无关个体作ＡＢＯ基因型分型。结果ＡＢＯ的６种基因型分型明确，１２５例汉族人ＡＢＯ基因型分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡。结论该方法操作简单，分型明确，重复性好，适用于法医学鉴定。
|
| 关 键 词： | ABO血型 基因型 聚合酶链反应 限制酶 |
Detection of ABO genotypes by simultaneous PCR-RFLP method |
| |
| YANG Qingen,ZHU Chuanhong.Detection of ABO genotypes by simultaneous PCR-RFLP method[J].Chinese Journal of Medical Genetics,1999,16(2):110-112. |
| |
| Authors: | YANG Qingen ZHU Chuanhong |
| |
| Institution: | Department of Forensic Medicine, Tongji Medical University, Wuhan, 430030, P. R. China. fywz@tjmu.edu.cn |
| |
| Abstract: | OBJECTIVE: To study human ABO genotyping by means of multiplex PCR and restriction fragment length polymorphism. METHODS: Two specific fragments of ABO gene were simultaneously amplified in a single tube, and then the double restriction digestion with RE Kpn I and Alu I was performed in the same tube. The amplified products were analyzed by PAGE and silver staining. A total of 125 Han unrelated individuals living in Wuhan were genotyped. RESULTS: Six genotypes of ABO were detected and the distribution was in good agreement with Hardy-Weinberg equilibrium. CONCLUSION: The results demonstrate that PCR-RFLP based approach is convenient, reliable and should be of value in forensic application. |
| |
| Keywords: | ABO blood group Genotype Polymerase chain reaction Restriction fragment polymorphism |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
