外周血人类斯钙素-1基因表达与乳腺癌微转移的关系

目的:检测乳腺癌患者外周血、肿瘤组织中hSTC-1mRNA的表达,判断外周血微转移状况,探讨微转移与肿瘤恶性程度之间的关系。方法:采用RT-PCR法检测乳腺癌组织、外周血中hSTC-1的mRNA表达,用免疫组化法检测乳腺肿瘤组织中ER、PR及VEGF的表达。结果:hSTC-1mRNA在乳腺癌患者癌组织中的阳性率为93.3%(28/30),在外周血中的阳性率为37.3%(19/51);17例非肿瘤成年女性患者外周血中未检测到hSTC-1的mRNA。血液中的hSTC-1的mRNA表达与多个临床病理因子有相关性(P<0.05),包括:肿瘤大小、腋淋巴结转移、TNM分期、ER、VEGF等;结论:乳腺癌患者外周血检测到hSTC-1基因的表达,说明乳腺癌已发生血行微转移,乳腺癌患者外周血hSTC-1的mRNA表达与多个临床病理因子有关;这些因子决定乳腺癌的预后,因此hSTC-1可以作为检测乳腺癌外周血微转移的一种新的分子标记。

Expression of hSTC-1 gene in peripheral blood and its correlation with micrometastases of breast cancer

Objective:To detect the expression of human stanniocalcin-1(hSTC-1) mRNA in peripheral blood and tumor tissues of breast cancer patients,determine micrometastases in peripheral blood,and study the relationship between the micrometastases and malignant degree of breast cancer.Methods:The expression of hSTC-1 mRNA in peripheral blood and breast cancer tissues was detected by RT-PCR.The expression of estrogen receptor(ER),progesterone receptor(PR),and vascular endothelial growth factor(VEGF) in breast cancer tissues were detected by immunohistochemical method.Results:Expression of hSTC-1 mRNA was identified in breast cancer tissues from 28 of 30 patients(93.3%) and in peripheral blood from 19 of 51 patients(37.3%).Expression of hSTC-1 mRNA was not detected in the peripheral blood from 17 women without breast cancer.Expression of hSTC-1 mRNA in the peripheral blood significantly correlated with multiple clinicopathological factors(P<0.05),including tumor size,metastasis of lymph nodes,TNM stage,and the expression of ER and VEGF.Conclusion:The expression of hSTC-1 mRNA in the peripheral blood of breast cancer patients suggests the occurrence of micrometastasis.It significantly correlates with multiple clinicopathological factors which determined the prognosis of breast cancer.So hSTC-1 is proposed as a new marker for detecting micrometastasis of breast cancer in the peripheral blood.