| 酸性染料比色法测定钩藤的总生物碱含量 |
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| 引用本文： | 曾常青,罗北亮. 酸性染料比色法测定钩藤的总生物碱含量[J]. 中药材, 2007, 30(8): 1021-1024 |
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| 作者姓名： | 曾常青 罗北亮 |
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| 作者单位： | 广东药学院中药学院,广东广州,510006 |
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| 摘 要： | 目的：探讨钩藤中总生物碱含量测定的方法。方法：采用酸性染料比色法测定钩藤中总生物碱含量。结果：酸性染料比色法的显色条件为pH3．6缓冲液5．0ml，溴甲酚绿液5．0ml；氯仿萃取三次，每次4．0ml，每次萃取振摇2min，静置5min。钩藤碱在6．018μg-108．324μg范围内呈线性关系，回收率平均值97．19％，RSD=1．34％（n=6）。结果：该方法灵敏度高，准确度好、重现性好。
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| 关 键 词： | 酸性染料比色法 钩藤 总生物碱 |
| 文章编号： | 1001-4454（2007）09-1021-04 |
| 修稿时间： | 2007-02-15 |
Contents Mensuration of Total Alkaloid in Uncaria rhynchophylla by Acid Dye Colorimetry |
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| ZENG Chang-qing,LUO Bei-liang. Contents Mensuration of Total Alkaloid in Uncaria rhynchophylla by Acid Dye Colorimetry[J]. Journal of Chinese medicinal materials, 2007, 30(8): 1021-1024 |
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| Authors: | ZENG Chang-qing LUO Bei-liang |
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| Affiliation: | Guangdong College of Pharmacy, Guangzhou 510006, China |
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| Abstract: | OBJECTIVE: To investigate the method of determination of total alkaloids Uncaria rhynchophylla. METHODS: The Contents of total Alkaloid were determined by Acid dye Colorimetry. RESULTS: Acid dye color conditions: pH3.6 buffer 5.0 ml, bromocresol green liquid 5.0 ml; chloroform extraction three times, each time was exeracted for 2 minutes, put it aside for at least 5 minutes for the determination of the best method. Rhynchophylline 6.018 microg - 108.324 microg in the linear range, Recoveriys rate was 97.19%, RSD was 1.34% (n = 6). CONCLUSION: The method is simple, highly sensitive and reproducible. |
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| Keywords: | Acid dye colorimetry Uncaria rhynchophylla Total Alkaloids |
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