不同镀膜磁体对牙龈成纤维细胞活性及mTOR表达的影响

目的评价各种镀膜钕铁硼磁体的生物相容性。方法将托槽、不同镀膜及未镀膜磁体加入DMEM中制备成浸提液,分别以5%和10%浓度加入培养的大鼠牙龈成纤维细胞,以AlamarBlueTM法测细胞活性。对加入10%浸提液的培养细胞做雷帕霉素靶蛋白(mTOR)的免疫印记分析。结果结果显示5%托槽、镀氮化钛磁体组的AlamarBlue还原率分别为63.0±3.4%、66.5±3.0%,与对照组64.2±3.4%无差异(P>0.05);10%托槽、镀氮化钛磁体组的还原率分别为62.0±2.17%、64.9±0.5%,与对照组比较65.7±1.0%无差异(P>0.05);而其余各组培养细胞的AlamarBlue还原率与对照组有显著性差异(P<0.05)。免疫印记结果显示,mTOR表达的平均灰度值托槽组77.0±2.85与镀氮化钛组81.2±2.60无显著性差异(P>0.05);镀镍组40.5±1.94,镀铜组15.0±2.01和未镀膜磁体组24.7±2.10与托槽组差异显著(P<0.01)。结论镀氮化钛钕铁硼磁体具有良好的生物相容性。

The Effects of Different Coating Magnets on the Activity and mTOR Expression of Rat Gingival Fibroblast Cell

Objective To investigate the biocompatibility of different coating NdFeB magnets. Methods Brackets ,different coating magnets and bare magnets were incubated in DMEM to make solutions, 5%and 10% those solutions were added into cultured rat gingival fibroblast cells. AlamarBlue  TM was used to examine the changes of cell activity . Western blotting was used to examine the expression of mammalian target of rapamycin( mTOR) in cells cultured with 10% solutions .Results The percentage of reduced AlamarBlue in 5% brackets, titanium-nitride magnets groups were 63.0±3.4%,66.5±3.0%,without obvious difference compared with control group 64.2±3.4% (P> 0.05). The percentage of reduced AlamarBlue in 10% bracket, titanium-nitride magnet groups were 62.0±2.17%,64.9±0.5%,without obvious difference compared with control group 65.7±1.0% (P> 0.05). The percentage of reduced AlamarBlue in nickle, copper, bared magnet groups had significant difference compared with control groups(P< 0.05).Western blotting showed that there was no significant difference for average gray value of mTOR between bracket 77.0±2.85 and titanium-nitride 81.2±2.60 (P<0.05),but there was significant difference in nickle group 40.5±1.94,copper group 15.0±2.01, bared magnet group 24.7±2.10 compared with bracket group(P<0.01).Conclusion Magnet coated titanium nitride has good biocompatibility.