| 基因芯片筛选结肠癌伊立替康耐药相关基因 |
| |
| 引用本文： | 陈曦,陈向征,刘明,郎楠,唐秋琳,陈济,赵晓斐,毕锋. 基因芯片筛选结肠癌伊立替康耐药相关基因[J]. 四川大学学报(医学版), 2011, 42(1): 15-18,36 |
| |
| 作者姓名： | 陈曦 陈向征 刘明 郎楠 唐秋琳 陈济 赵晓斐 毕锋 |
| |
| 作者单位： | 四川大学华西医院肿瘤中心腹部肿瘤科,成都,610041 |
| |
| 摘 要： | 目的 应用基因芯片技术进行结肠癌伊立替康耐药相关基因表达谱差异分析.探讨伊立替康结肠癌耐药发生机制.方法 分别抽提人结肠癌细胞系SW480及其伊立替康耐药细胞系SW480/CPT总RNA,逆转录合成相应以Cy3标记的cRNA做探针,在Agilent基因芯片上杂交,Axon 4000B扫描仪扫描芯片荧光信号图像.Gene...
|
| 关 键 词： | 基因芯片 伊立替康 耐药 结肠癌 基因表达 |
Analysis of Gene Expression Patterns in an Irinotecan-resistance Colon Cancer Cell by cDNA Microarray |
| |
| CHEN Xi,CHEN Xiang-zheng,LIU Ming,LANG Nan,TANG Qiu-lin,CHEN Ji,ZHAO Xiao-fei,BI Feng. Analysis of Gene Expression Patterns in an Irinotecan-resistance Colon Cancer Cell by cDNA Microarray[J]. Journal of Sichuan University. Medical science edition, 2011, 42(1): 15-18,36 |
| |
| Authors: | CHEN Xi CHEN Xiang-zheng LIU Ming LANG Nan TANG Qiu-lin CHEN Ji ZHAO Xiao-fei BI Feng |
| |
| Affiliation: | CHEN Xi,CHEN Xiang-zheng,LIU Ming,LANG Nan,TANG Qiu-lin,CHEN Ji,ZHAO Xiao-fei,BI Feng. Department of Abdominal Cancer,Cancer Center,West China Hospital,Sichuan University,Chengdu 610041,China |
| |
| Abstract: | Objective To study the mechanism of irinotecan-resistant colon cancer by analyzing the differential gene expression patterns with cDNA microarray.Methods Total RNA was purified from irinotecan-sensitive colonic cancer cell line,SW480 and its irinotecan-resistance cell line,SW480/CPT.The cRNA retro-transcribed from RNA were labeled with Cy3 fluorescence as probes.The probes were hybridized with Agilent gene chips and the fluorescence images of the chips were obtained with Axon 4000B scanner as well as analyz... |
| |
| Keywords: | cDNA microarray Irinotecan Drug resistance Colon cancer Gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
