HPLC测定金莲花制剂中荭草苷和牡荆苷的含量

目的 :建立金莲花制剂中荭草苷和牡荆苷的含量测定方法。方法 :采用高效液相色谱法 ,使用HypersilC18ODS色谱柱 ,流动相乙腈 1%醋酸溶液 (15∶85 ) ,流速 1.0mL·min^-1,检测波长 340nm。结果 :荭草苷在 8.42～84.2 μg·mL^-1呈良好的线性关系 (r=0.9999) ,平均回收率为 98 3% ,RSD =0.8% ;牡荆苷在 8.07～ 80.7μg·mL^-1呈良好的线性关系 (r=0.9999) ,平均回收率为 102.4 % ,RSD =1.7%。结论 :本法灵敏、准确、专属性强 ,重现性好 ,可作为金莲花制剂的质量控制方法。

Determination of orientin and vitexin in Trollius chinesis preparation by HPLC

OBJECTIVE: To establish a HPLC method for determining orientin and vitexin in Trollius chinesis preparation. METHOD: The separation was carried out on Hypersil ODS column with a mixture of acetonitrile-1% acetic acid solution (15:85) as the mobile phase, at a flow rate of 1.0 mL x min(-1). The detection wavelength was set 340 nm. RESULT: Orientin showed a good linear relationship in the range of 8.42-84.2 microg x mL(-1) (r = 0.9999) and the average recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07-80.7 microg x mL(-1) (r = 0.9999) and the average recovery is 102.4%. RSD is 1.7%. CONCLUSION: The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation.