Genistein对小鼠小肠上皮细胞照射后再增殖和TGF-α、EGF-R表达的影响

目的研究Ｇｅｎｉｓｔｅｉｎ对小鼠小肠上皮细胞受照射后再增殖和ＴＧＦ－α、ＥＧＦ－Ｒ表达的影响。方法实验鼠分两组：一组为对照组，单纯一次接受全身吸收剂量为５Ｇｙ的照射；另一组为用药组，照射剂量与方法同对照组，在照射后不同时间注射Ｇｅｎｉｓｔｅｉｎ。在照射后不同时间内杀死小鼠取出空肠，计数隐窝细胞数和分裂指数。并测定隐窝区和绒毛区ＴＧＦα和ＥＧＦＲ的表达。结果与对照组比较，用药组小鼠小肠隐窝细胞的分裂指数和细胞数上升幅度较低，用药组ＴＧＦα在隐窝区表达持续升高，而ＥＧＦ－Ｒ在隐窝区的表达则较对照组低。结论Ｇｅｎｉｓｔｅｉｎ能明显抑制小鼠小肠上皮细胞受照射后的再增殖，这种影响可能是通过作用于ＥＧＦ－Ｒ的表达起作用。

Effects of genistein on repopulation of mouse jejunum crypts and expression of TGF- and EGF-R following irradiation

Objective To study the effects of genistein on the repopulation of mouse jejunum crypts and expression of TGF- and EGF-R following irradiation. Methods C3Hf/kam mice were treated by radiotherapy plus genistein as the study group or treated by radiotherapy only as the control group.Using jejunum as material,the number of cryptal cells and mitotic index were measured at different time intervals after irradiation.At the same time the expression of TGF- and EGF-R were also tested by immunohistochemical method.HZ] Results Comparied with the control group,the number of cryptal cells and mitotic index in the study group were decreased on days 34 after irradiation.The expression of TGF- in crypts kept a high level while the expression of EGF-R in crypts decreased after irradiation in the study group. Conclusion The results indicate that genistein can suppress the repopulation of mouse jejunum crypts after irradiation while TGF- and EGF-R play important roles in repopulation of mouse jejunum crypts after irradiation.