| 激活素A及卵泡抑素基因在糖尿病大鼠肾脏表达的实验研究 |
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| 引用本文： | 任小军,柳刚,关广聚.激活素A及卵泡抑素基因在糖尿病大鼠肾脏表达的实验研究[J].医学理论与实践,2011,24(12):1365-1367. |
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| 作者姓名： | 任小军 柳刚 关广聚 |
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| 作者单位： | 1. 山西省人民医院肾内科,山西省太原市,030012
2. 山东大学第二医院肾内科 |
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| 基金项目： | 国家教育部高等学校博士学科点基金新教师基金资助 |
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| 摘 要： | 目的:探讨激活素A(ACT-A)及卵泡抑素(FS)基因在糖尿病大鼠肾脏的表达及可能机制。方法:雄性Wistar大鼠,随机分成对照组(NC)和糖尿病组(DM),腹腔注射STZ诱导糖尿病模型。各组分别在术后4、8、12和16周处死6只大鼠。观察各组肾重/体重(KW/BW)、24h尿白蛋白排泄率(AER)及肌酐清除率(Ccr)的变化。real time-PCR检测各组肾组织ACT-A及FS基因的表达。结果:与NC组相比,DM组大鼠从第8周末开始KW/BW、AER及Ccr均显著上升(P<0.01),16周末时达峰值。DM组大鼠从第4周末开始ACT-A mRNA表达逐渐增加,第12周末时达峰值(P<0.01)。FS mRNA在NC组各时间点大鼠肾组织大量表达,而在DM组其表达量呈逐渐下降趋势,第16周时只能检测到基础量表达。结论:ACT-A及FS表达失衡在DN的进行性发展中发挥了重要作用。
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| 关 键 词： | 糖尿病肾病 激活素A 卵泡抑素 |
Experimental Research of Activin A and Follistatin Gene Expression in the Kidney of Diabetic Rats |
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| REN Xiaojun,LIU Gang,GUAN Guangju.Experimental Research of Activin A and Follistatin Gene Expression in the Kidney of Diabetic Rats[J].The Journal of Medical Theory and Practice,2011,24(12):1365-1367. |
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| Authors: | REN Xiaojun LIU Gang GUAN Guangju |
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| Institution: | REN Xiaojun,LIU Gang,GUAN Guangju.Department of Nephrology,The People's Hospital of Shanxi Province,Taiyuan City,Shanxi Province 030012 |
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| Abstract: | Objective:To explore the effect of activin A and follistatin in diabetic nephropathy(DN) through observing their gene expression in the kidney of diabetic rats.Methods:Male Wistar rats were randomized into normal control group(NC)and diabetes mellitus group(DM).Diabetes was induced by intraperitoneal injection of streptozotocin(STZ).Six rats were respectively sacrificed 4,8,12 and 16 weeks after model establishment in each group.The changes of kidney weight/body weight(KW/BW),urine albumin excretion rate(AE... |
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| Keywords: | Diabetic nephropathy Activin A Follistatin |
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