MSP法检测胃癌的PTEN基因甲基化改变

目的:检测胃癌中抑癌基因PTEN启动子区域甲基化状况,并探讨其甲基化改变的特点与临床病理特征的关系。方法:采用甲基化特异的PCR法(MSP)检测胃癌组织及相应癌旁组织(各35例)中PTEN启动子区域甲基化状态。结果:35例癌旁正常胃组织未发现有PTEN基因启动子的甲基化,16/35例胃癌组织检测到PTEN基因启动子的甲基化,癌组织PTEN基因启动子甲基化率显著增高(P<0.05)。有淋巴结转移的19例胃癌组织中,有12例PTEN基因启动子甲基化,有淋巴结转移的PTEN基因启动子甲基化显著高于无淋巴结转移组(P<0.05)。结论:PTEN基因启动子的甲基化与胃癌的发生、转移相关。甲基化特异的PCR法(MSP)是检测胃癌抑癌基因PTEN启动子区域甲基化状况的一种较新且特异的实验方法,可用于胃癌的辅助诊断和预后判断。

Detection of Promoter Methylation of PTEN Gene in Gastric Carcinoma by MSP

Objective: To detect methylation status of the 5'CpG island locating in the promoter region of PTEN gene in gastric carcinoma, and to analyze the relationship between the aberrant methylaion of PTEN gene and clinical manifestation. Methods: By methlation-specific PCR technique (MSP), specimens (tumor and adjacent normal tissues) from 35 gastric carcinoma patients were analyzed to detect methylation status of PTEN promoter region. Results: No methylation of PTEN promoter was found in adjacent normal tissues, while hypermethlation of PTEN gene in promoter region were detected in 16 cases of gastric carcinoma(16/35, 45.7%), and the rate of methylaion of PTEN promoter in gastric carcinoma was higher than that of in adjacent normal tissues(P<0.05). In 19 cases with lymphnode metastasis, 12 were found with PTEN promoter methylation. A significant difference was noted between the two groups (P<0.05). Conclusion: Methylation of PTEN promoter is correlated to oncogenesis and metastasis of gastric carcinoma. Methylation specific polymerase chain reaction (MSP) is a novel and specific method for methylation detection around the promoter region of PTEN gene, which can be helpful in diagnosis and prognosis of gastric carcinoma clinically.