四环素和多西环素对人牙周韧带细胞生物学活性的影响

目的探讨四环素及多西环素对体外培养的人牙周韧带成纤维细胞(HPDLCs)的生物学作用。方法将不同浓度的2种药物(1、5、20、100、500、2500滋g/ml)加入体外培养的HPDLCs中,共孵育2d后,在倒置显微镜下观察其对细胞形态的影响,并用MTT法、考马司亮蓝法及3H-TdR掺入法,分别检测其对细胞的增殖活性、蛋白合成及DNA合成的影响。结果在1～100滋g/ml的浓度范围内,2种药物对细胞形态无影响。在20～100滋g/ml的浓度范围内,四环素显著促进HPDLCs的增殖及生物合成(P﹤0.01),而多西环素只在20滋g/ml时,能显著促进细胞DNA的合成(P﹤0.01)。当2种药物的浓度增至2500滋g/ml时,不仅使细胞的镜下形态发生明显改变,而且严重抑制细胞的生物学活性。结论在合适的浓度范围内,四环素能显著促进HPDLCs的增殖及生物合成,多西环素的促进作用不明显,而浓度过高时两者均具有细胞毒性。

Biological Effects of Tetracycline and Doxycycline on Cultured Human Periodontal Ligament Cells

OBJECTIVE: To explore the biological effects of tetracycline and doxycycline on cultured human periodontal ligament cells(HPDLCs). METHODS: Increasing concentrations of two drugs(1,5,20,100,500,2500 microg/ml)were added to the medium of cultured HPDLCs separately. After being co-incubated for 2 days, cell morphology was observed under reverse microscope; meanwhile, cell proliferation activity was assayed using MTT, the total amount of protein was detected by Coumassie Bright Blue method and DNA synthesis was measured by 3H-TdR. RESULTS: Over a concentration range of 1 to 100 microg/ml, cell morphology was normal. Moreover, at a concentration range of 20 to 100 microg/ml, tetracycline significantly enhanced the proliferative activity and biosynthesis of HPDLCs (P<0.01) while doxycycline significantly promoted DNA synthesis only at the concentration of 20 microg/ml. However, higher concentration of the two drugs (2500 microg/ml) not only changed cell morphology under microscope, but also significantly inhibited cellular activity. CONCLUSION: The results suggested that proper doses of tetracycline could promote proliferation and biosynthesis of HPDLCs,while doxycycline had limited effects on biobehavior of HPDLCs. Nevertheless, higher concentrations of the two drugs both had cytotoxic effect.