补阳还五汤对高脂血症模型大鼠脂质代谢及肝组织的影响

目的:探讨补阳还五汤对高脂血症模型大鼠脂质代谢及肝组织的影响。方法:将雄性SD大鼠随机分为空白对照组、模型组、阳性对照组(辛伐他汀0.004 g/kg)和补阳还五汤低、高剂量组(3.5、14.0 g/kg,按生药总量计),每组10只。除空白对照组大鼠灌胃水外,其余各组大鼠均按体质量灌胃脂肪乳剂复制高脂血症模型。从造模第21天开始,各组大鼠上午继续灌胃水或脂肪乳剂,下午灌胃水或相应药物,连续20 d。末次给药后,检测各组大鼠血浆中三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、卵磷脂胆固醇脂酰基转移酶(LCAT)、磷脂转移蛋白(PLTP)、肝脂酶(HL)含量以及肝细胞中脂蛋白脂肪酶(LPL)、微粒体三酰甘油转运蛋白(MTP)、3-羟基-3-甲基戊二酸单酰辅酶A(HMG-CoA)还原酶的含量,并观察各组大鼠肝组织的病理形态学变化。结果:与空白对照组比较,模型组大鼠TG、TC、LDL-C、ALT、HMG-CoA还原酶含量均显著升高(P<0.05或P<0.01),HL、LPL、MTP含量均显著降低(P<0...

Effects of Buyang Huanwu Decoction on Lipid Metabolism and Hepatic Tissue in Hyperlipemia Model Rats

OBJECTIVE:To investigate the effects of Buyang huanwu decoction on lipid metabolism and hepatic tissue in hyperlipemia model rats.METHODS:Male SD rats were randomly divided into blank control group,model group,positive control group(simvastatin 0.004 g/kg),Buyang huanwu decoction low-dose and high-dose groups(3.5,14.0 g/kg,by crude drugs),with 10 rats in each group.Except that blank control group was given water intragastrically,hyperlipidemia mode of other groups was established by intragastric administration of fat emulsion according to the body weight.From the 21 st day after modeling,the rats in each group were given water or fat emulsion in the morning and water or corresponding drugs in the afternoon for consecutive 20 days.After last medication,the contents of TG,TC,LDL-C,ALT,AST,LCAT,PLTP and HL in plasma were determined in each group;the contents of LPL,MTP and HMG-CoA redutase in liver tissue were also determined.The pathological changes of liver tissue were observed in each group.RESULTS:Compared with blank control group,the contents of TG,TC,LDL-C,ALT and HMG-CoA reductase were increased significantly in model group(P<0.05 or P<0.01),while the contents of HL,LPL and MTP were decreased significantly(P<0.01).Compared with model group,the contents of TG,TC,LDL-C and HMG-CoA reductase in administration groups as well as the contents of ALT,AST in Buyang huanwu decoction low-dose and high-dose groups were decreased significantly(P<0.05 or P<0.01);the contents of HL and MTP in administration groups as well as the content of LPL in positive control group were increased significantly(P<0.05 or P<0.01).Pathological sections showed that the hepatocytes of model group were arranged irregularly,and there was obvious cell steatosis.The hepatocytes in positive control group and Buyang Huanwu decoction high-dose group had almost no abnormal change compared with blank control group,and there was a small amount of slight cell steatosis in Buyang Huanwu decoction low-dose group.CONCLUSIONS:Buyang huanwu decoction has a significant lipid-lowering effect on hyperlipemia model rats,which may be related to the regulation of lipid absorption,transport and metabolism by regulating the expression of lipid metabolism related enzymes and transporter.