Bucillamine improves hepatic microcirculation and reduces hepatocellular injury after liver warm ischaemia-reperfusion injury |
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Authors: | Sameer P Junnarkar Niteen Tapuria Neelanjana Dutt Barry Fuller Alexander M Seifalian Brian R Davidson |
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Institution: | 1.University Department of Surgery, Royal Free Campus, Royal Free and University College Medical School, UCL and Royal Free hospital, London, UK;2.Department of Pathology, King''s College Hospital, London, UK |
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Abstract: | Background:Liver transplantation and resection surgery involve a period of ischaemia and reperfusion to the liver which initiates an inflammatory cascade resulting in liver and remote organ injury. Bucillamine is a low-molecular-weight thiol antioxidant that is capable of rapidly entering cells.Methods:The effect of bucillamine was studied in a rat model of liver ischaemia–reperfusion injury with 45 min of partial (70%) liver ischaemia and at 3 and 24 h of reperfusion. Controls included ischaemia-reperfusion (I/R) only, sham and bucillamine alone (without ischaemia reperfusion). Liver injury was assessed by serum transaminases (AST and ALT). Sinusoidal blood flow and hepatocyte apoptosis were measured using intravital microscopy (IVM).Results:The hepatocellular injury of I/R produced a markedly elevated serum AST which was reduced with bucillamine (2072.5 ± 511.79 vs. 932 ± 200.8, P < 0.05) at 3 h reperfusion. Bucillamine treatment with I/R also increased parenchymal blood flow red blood cell (RBC) velocity 242.66 ± 16.86 vs. 181.11 ± 17.59, at the end of 3 h of reperfusion) and reduced hepatocyte necrosis/apoptosis at 3 h as well as 24 h (P > 0.001).Conclusion:Bucillamine reduces the hepatocellular injury of liver ischaemia reperfusion and improves parenchymal perfusion. |
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Keywords: | liver ischaemia-reperfusion injury intravital microscopy antioxidants Bucillamine |
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