正肝化瘀方对肝纤维化大鼠肝组织MMPs和TIMPs蛋白表达的影响

目的：观察正肝化瘀方对肝纤维化大鼠肝组织MMPs和TIMPs蛋白表达的影响，探讨其抗肝纤维化的作用机制。方法：将36只雄性SD大鼠随机分为正常组、模型组与正肝化瘀方组，对正肝化瘀方组大鼠采用四氯化碳＋高脂低蛋白饮食诱导肝纤维化模型，造模当天即以正肝化瘀方溶液进行灌胃，6周，模型组和正常组大鼠予以等量生理盐水灌胃。采用蛋白印迹法分析各组大鼠肝组织α-SMA、TIMP-1和TIMP-2蛋白的表达，明胶酶图法检测各组大鼠肝组织MMP-2和MMP-9的活性。结果：模型组大鼠肝组织α-SMA蛋白表达明显增强，正肝化瘀方组大鼠α-SMA蛋白表达明显减弱；模型组大鼠肝组织MMP-2／9活性显著升高，正肝化瘀方组的MMP-2／9蛋白活性明显下降；模型组大鼠肝组织TIMP-1／2蛋白表达明显增强，正肝化瘀方组大鼠肝组织TIMP-1／2蛋白表达较模型组减弱，其TIMP-2蛋白表达减弱更为明显。结论：正肝化瘀方能显著下调α-SMA蛋白表达，降低MMP-2／9蛋白活性，下调MMP-2／9蛋白和TIMP-1／2蛋白的表达．促进ECM（细胞外基质）的降解，抑制肝纤维化的形成，促进肝纤维化的逆转。

Effect of Zhenggan Huayu recipe on the expressions of MMPs and TIMPs in the hepatic tissues of rats with hepatic fibrosis

To observe the effect of Zhenggan Huangu recipe on the expressions of MMPs and TIMPs in the hepatic tissues of rats with hepatic fibrosis and probe its mechanism of anti-hepatic fibrosis. Methods： A complex of carbonate chloride, high fat and low protein was used to induce animal models of hepatic fibrosis. All the rats were randomly divided into 3 groups, normal group, model group and treatment group. Rats in the treatment group were given Zhenggan Huayu recipe through the gastric syringe for 6Ws. Rats in the model group were given normal saline of the same volume, α-SMA, TIMP-1 and TIMP-2 were tested through western blotting. The activities of MMP-2 and MMP-9 were detected through zymography. Results： The expressions of α-SMA were significantly increased in the model group while decreased in the treatment group. The same results coule also be seen with regard to the activities of MMP-2 and 9. Conclusion： Zhenggan Huayu recipe can downgrade the expressions of α-SMA, TIMP-1 and 2 and decrease the activities of MMP-2 and 9, accelerate the retrogradation of ECM, inhibit the formation of hepatic fibrosis, and in a way reverse hepatic fibrosis.