| 应用GFP基因转染示踪BMSCs在组织工程下颌骨内的分化 |
| |
| 引用本文： | 袁捷,韦敏,祁佐良,陈瑾君,崔磊,曹谊林.应用GFP基因转染示踪BMSCs在组织工程下颌骨内的分化[J].中国口腔颌面外科杂志,2010,8(2):165-170. |
| |
| 作者姓名： | 袁捷 韦敏 祁佐良 陈瑾君 崔磊 曹谊林 |
| |
| 作者单位： | 上海市组织工程研发中心,上海,200235;上海交通大学医学院附属第九人民医院,整形外科,上海市组织工程实验室,上海,200011 |
| |
| 基金项目： | 国家“973”组织工程基本科学问题项目(2005CB522700); 国家“863”组织工程化骨构建技术研究与产品开发(2002AA205011); 上海市教育委员会自然科学类科研创新项目(08YZ49)~~ |
| |
| 摘 要： | 目的：应用绿色荧光蛋白（GFP）标记技术,观察组织工程化下颌骨形成过程中种子细胞的变化与转归。方法：用GFP反转录病毒载体与疱疹性口炎病毒糖蛋白G基因（VSV-G）蛋白重组形成假病毒,高效转染犬骨髓间质干细胞（bone marrow stromal cells,BMSCs）,将其接种于珊瑚,形成细胞、材料复合物,植入修复犬下颌骨标准缺损。术后32周取材,进行大体观察,组织学观察组织结构,免疫组化检测GFP蛋白表达情况。结果：32周后,大体可见组织工程化下颌骨形成,Van Gieson染色示新生骨大部分为成熟骨质,免疫组化显示新骨表面有GFP阳性成骨细胞,连接处无GFP阳性细胞,,珊瑚大部分降解。结论：组织工程下颌骨结构与正常皮质骨类似,新生组织表达GFP,证实组织工程下颌骨组织的形成来源于植入细胞。
|
| 关 键 词： | 疱疹性口炎病毒糖蛋白G基因 绿色荧光蛋白 组织工程 下颌骨 |
A pilot study of using GFP labelling to monitor tissue engineered bone formation in repairing of canine segmental mandibular defects |
| |
| YUAN Jie,WEI Min,QI Zuo-liang,CHEN Jin-jun,CUI Lei,CAO Yi-lin.A pilot study of using GFP labelling to monitor tissue engineered bone formation in repairing of canine segmental mandibular defects[J].China Journal of Oral and Maxillofacial Surgery,2010,8(2):165-170. |
| |
| Authors: | YUAN Jie WEI Min QI Zuo-liang CHEN Jin-jun CUI Lei CAO Yi-lin |
| |
| Institution: | YUAN Jie,WEI Min,QI Zuo-liang,CHEN Jin-jun,CUI Lei,CAO Yi-lin.(Department of Plastic , Reconstructive Surgery,Ninth People\'s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Tissue Engineering Center.Shanghai 200011,Shanghai Tissue Engineering Research , Development Center.Shanghai 200235,China) |
| |
| Abstract: | PURPOSE:To reconstruct tissue engineered mandible with cultured bone marrow stromal cells(BMSCs) and trace it by green fluorescent protein(GFP) gene transfer.METHODS:The pseudotyped RV-GFP expression vector was used to infect canine BMSCs directly.GPF-labeled BMSCs were seeded within coral to form a cell-scaffold construct,then implanted to repair canine 3cm segmental mandibular defects.New bone was harvested at 32 weeks post-operation and evaluated by gross view,Van Gieson staining and GFP immunohistochemi... |
| |
| Keywords: | Vesicular stomatitis virus G glycoprotein Green fluorescent protein Tissue engineering Mandible |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
