突变型P53与MDR在膀胱移行细胞癌中表达的意义

目的探讨膀胱移行细胞癌(BTCC)中MDR的表达产物P-gp及P53表达的意义。方法利用免疫组织化学链霉菌素-生物素(S-P)法测83例BTCC的P-gp和P53的表达。结果在83例膀胱移行细胞癌中P-gp阳性表达率为71%,P53阳性表达率为51%;P-gp阳性表达在病理分级、临床分期及预后中差异无统计学意义(P>0.05);P53随着病理分级和临床分期而增高,差异均有统计学意义(P<0.05);P53阳性表达复发组高于无复发组(P<0.01)。结论检测P-gp可指导BTCC的化疗药物选择,P53高表达与BTCC的进展有关,P-gp、P53高表达者预后及疗效差,P53可作为BTCC独立的预后因素。

Significance of expression of mutant P53 and MDR in bladder transitional cell carcinoma

Objective To explore the significance of expression of P-gp and mutant p53 in bladder transitional cell carcinoma.Methods The expression of P-gp and p53 gene in specimens of 83 cases of patients with bladder transitional cell carcinoma were studied by immunohistochemical method.Results The positive expression rate of P-gp gene and mutant p53 gene was 71% and 51% respectively in specimens of 83 cases.The level of P-gp has no significantly statistical difference with the tumor pathological grade,clinical stage and prognosis(P>0.05).The expression of p53 was higher in high grade tumors.It had a significantly statistical difference(P<0.05).The mutant p53 positive rates were higher in recurrent patients(P<0.01).Conclusion To examine P-gp is beneficial to selection of bladder carcinoma chemotherapeutic medicines.The mutant p53 gene may be involved in tumor progression in urotholied neoplasis.Overexpression of P-gp and mutant p53 of the tumors suggests that the patients have a worse prognosis and poor reaction to the therapy.The mutant p53 gene could be taken as an independent prognostic factor for tumor progression in BTCC.