| 原位杂交法检测艾滋病尸检组织中病毒核酸 |
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| 引用本文： | 周先志,王松山,王凝芳,孙中和,朱纯吾,朱关福.原位杂交法检测艾滋病尸检组织中病毒核酸[J].中华传染病杂志,1994(3). |
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| 作者姓名： | 周先志 王松山 王凝芳 孙中和 朱纯吾 朱关福 |
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| 作者单位： | 北京解放军第三○二医院，解放军军事医学科学院 |
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| 摘 要： | 对曾用免疫组织化学方法检测了人免疫缺陷病毒１型（ＨＩＶ－１）抗原的１例艾滋病尸检病例的脑、肝、肾、心、肺、胰、肠、脾、胸腺、淋巴结和兰尾等组织标本，采用地高辛标记的ＨＩＶ－１ｃＤＮＡ探针检测病毒ＲＮＡ。结果发现在脑组织神经胶质细胞和星形细胞，肠上皮细胞以及淋巴结、脾和胸腺的淋巴细胞ＨＩＶ－１ＲＮＡ阳性。
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| 关 键 词： | 艾滋病，人免疫缺陷病毒1型核酸，原位杂交 |
DETECTION OF HIV-1 RNA IN TISSUE SECTIONO OF A CHINESE PATIENT WITH AIDS BY IN SITU HYBRIDIZATION |
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| Zhou Xian-zhi,Wang Song-san,Wang Ning-fang,et al..DETECTION OF HIV-1 RNA IN TISSUE SECTIONO OF A CHINESE PATIENT WITH AIDS BY IN SITU HYBRIDIZATION[J].Chinese Journal of Infectious Diseases,1994(3). |
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| Authors: | Zhou Xian-zhi Wang Song-san Wang Ning-fang |
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| Institution: | Zhou Xian-zhi,Wang Song-san,Wang Ning-fang,et al. 302 Hospital of Chinese PLA,Beijing,100039 |
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| Abstract: | Human immunodeficiency virus-1(HIV-1)gp120 and P17 antigens were studiedby immunohistochemistry in brain,liver,heart,lung,thymus,gut,spleen,appendix,kidney,lymph nodes and pancrease tissue from a chinese patient with acquiredmm modeficiency syndrome (AIDS). Among the tissue,HIV-1 RNA was detected by insitu hybridization with a digoxigenin-labeled HIV-1 cCNA probe. The results confirmedthe presence of HIV-1 RNA in glial cells and astrocytes of the brain,gut epithelialcells,lymphocytes from lymph nodes,spleen and thymus |
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| Keywords: | AIDS HIV-1 RNA In situ hybridization |
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