| Effects of platelet-activating factor on endothelial cell monolayer permeability under hydrostatic perfusion in vitro |
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| 引用本文： | 丁自强,李少华,周洪堂,吴中立. Effects of platelet-activating factor on endothelial cell monolayer permeability under hydrostatic perfusion in vitro[J]. 中国人民解放军军医大学学报, 1993, 0(3) |
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| 作者姓名： | 丁自强 李少华 周洪堂 吴中立 |
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| 作者单位： | Departments of Pathophysiology Second Military Medical University Shanghai，200433，Departments of Pathophysiology Second Military Medical University，Shanghai，200433，Departments of Computer，Second Military Medical University，Shanghai，200433，Departments of Pathophysiology Second Military Medical University，Shanghai，200433 |
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| 摘 要： | A simple and rapid method was established to study vascular permeability by in vitroperfused endothelial cell monolayers cultured on micropore filter membrane.It can be used todetermine filtration coefficient (K_f) to small molecules and osmotic reflection coefficient (σ) toproteins of the endothelial monolayer.Hanks' balanced salt solution (HBSS) or 5g/L albuminin HBSS was used to perfuse the confluent endothelial monolayer at the hydrostatic pressure of2.45kPa (25cm H_2O).Control K_f values were 10.1±0.75 and 3.6±0.75μl·min~(-1)·cm~(-2)·kPa(-1)(±,n=3) respectively for the perfusion of HBSS and albumin HBSS,suggesting that al-bumin may decrease endothelial monolayer permeability to water and small molecules.After ex-posure of endothelial monolayer to 10~(-8)mol/L platelet-activating factor (PAF) for 30min,K_fvalues increased to 193.1% and 133.3% respectively.Protein clearance rate (μl.min~(-1)·cm~(-2))and osmotic reflection coefficient of the control endothelial monolayer were 8.0±3.22 and 0.37±0.09 respectively and those of the PAF treated endothelial monolayer 12.2±2.95μl·min~(-1)·cm~(-2) and 0.18±0.06,revealing increased permeability to albumin.Computer-assisted imageprocessing demonstrated that PAF treatment decreased cell area while increased cell form factorand intercellular space,suggesting that endothelial cells retracted and rounded,which may bean important mechanism of PAF-induced increase of vascular permeability.
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Effects of platelet-activating factor on endothelial cell monolayer permeability under hydrostatic perfusion in vitro |
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| Ding Ziqiang Li Shaohua Zhou Hongtang Wu Zhongli Departments of Pathophysiology and Computer,Second Military Medical University,Shanghai. Effects of platelet-activating factor on endothelial cell monolayer permeability under hydrostatic perfusion in vitro[J]. Journal of Medical Colleges of PLA(China), 1993, 0(3) |
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| Authors: | Ding Ziqiang Li Shaohua Zhou Hongtang Wu Zhongli Departments of Pathophysiology Computer Second Military Medical University Shanghai |
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| Affiliation: | Ding Ziqiang Li Shaohua Zhou Hongtang Wu Zhongli Departments of Pathophysiology and Computer,Second Military Medical University,Shanghai,200433 |
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| Abstract: | |
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| Keywords: | platelet activating factor endothelium permeability image processing in vitro |
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