丝裂霉素C聚氰基丙烯酸正丁酯磁性纳米球对肝细胞L-02的生长抑制作用

目的　研究丝裂霉素C聚氰基丙烯酸正丁酯磁性纳米球 (MMC PBCA MNPS)、PBCA MNPS和MF(磁流体 )以及MMC对正常人肝细胞株 (L 0 2细胞 )生长抑制作用的影响。方法　用乳化聚合法分别制备了PBCA MNPS与MMC PBCA MNPS;采用MTT比色法研究了不同浓度的MMC PBCA MNPS、PBCA MNPS、MF与MMC对L 0 2细胞生长抑制作用的影响 ;用自动生化仪测定了乳酸脱氢酶 (LDH)活性。结果收稿日期 :2 0 0 4-0 2 -0 3 ,修回日期 :2 0 0 4-0 3 -19基金项目 :国家“863”资助项目 ( 2 0 0 2AA2 14 12 1) ;广东省攻关资助项目 ( 2 0 0 2C3 0 10 3 )作者简介 :任　非 ( 1974-) ,女 ,博士 ,研究方向 :生物药剂学 ,Tel:0 2 0 6164 1888 872 3 6,E mail:renfeisky @tom .com ;陈建海 ( 1947-) ,男 ,教授 ,博士生导师 ,研究方向 :生物高分子材料 ,生物药剂学不同浓度的MMC PBCA MNPS对L 0 2细胞的相对抑制率(RIR % )为 :10 0 %、15 1%、2 5 1%、32 1%、39 9%与 4 5 0 % ;不同浓度的PBCA MNPS的RIR %依次为 :1 8%、5 2 %、10 0 %、12 9%、15 1%与 2 9 9% ;不同MMC浓度的RIR %依次为 :2 2 1%、35 1%、4 6 1%、6 2 0 %、80 1%与 88 9%。MF不仅不抑制L 0 2细胞的生长 ,还有利于细胞的生长。按照相对生长率 (RGR % ) ,MMC PBCA M

Growth inhibition effect of mitomycin polybutylcyanoacrylate magnetic nanoparticles on L-02 cell

Aim To investigate the effects of growth inhibitio n of different concentrations of mitomycin polybutylcyanoacrylate magnetic nanpart icles(MMC-PBCA-MNPS), PBCA-MNPS and MF (magnetic fluids) and MMC on normal hu man liver cell strain (L-02 cells) in vitro.Methods MMC-P BCA-MNPS and PBCA-MNPS were prepared by emulsion polymerization method, respectively. The influences of growth inhibition of different concentrations of MMC-PBCA-MNPS,PBCA-MNPS, MF and MMC on L-02 cells were studied by MTT colorimetric assay; the activity of lactate dehydrogenase (LDH) was detected by automatic biochemy-instrument. Results The relative inhibition rates (RIR%) of different concentrations of MMC-PBCA-MNPS for L-02 cells were:10.0%,15.1%,25.1%,32.1%,39.9% and 45.0%;PBCA-MNPS: 1.8%,5.2%,10.0%,12.9%,15.1% and 29.9%;MMC: 22.1%,35.1%,46.1%,62.0%,80.1% and 88.9%.MF did not significantly inhibit the growth of L-02 cells, meanwhile they were in favor of the growth of L-02 cells.Judging by relative growth rate (RGR%), the cytotoxicities grade of MMC-PBCA-MNPS, PBCA-MNPS, MF and MMC for L-02 cells were 1, 1, 0 and 4 grade, respectively. The activities of LDH were as follow: MMC-PBCA-MNPS: 117.00±16.00～354.0±14.18; PBCA-MNPS: 106.00±13.00～186.00±15.72; MF: 35.00±12.49～34.00±6.56 and MMC: 377.00±10.82～471.00±9.85; and blank: 34.00±5.29, P<0.05;P<0.01.Conclusion The relative degree of growth inhibition for L-02 cell strain is as follows: MMC>MMC-PBCA-MNPS>PBCA-MNPS>MF.