聚合酶链反应检测HBVM阳性血清中HBV DNA的临床意义

应用聚合链反应对１４０例乙型肝炎病毒感染标志（ＨＢＶ Ｍ）阳性的慢性肼病患者血清中ＨＢＶ ＤＮＡ进行检测。结果发现：１．ＨＢＶ ＤＮＡ总阳性率为７２．８６％；ＢＨｓＡｇ、ＨＢｅＡｇ和抗ＨＢｃ阳性血清中ＨＢＶＤＮＡ阳性纺分别为７６．５６％、９６．１５％和７４．４２％；ＨＢｓＡｇ阴性血清中ＨＢＶＤＮＡ阳性率为３３．３３％，ＨＢｅＡｇ阴性血清中ＨＢＶＤＮＡ阳性率为５９．０９％。２．各ＨＢＶ感染模式中，Ｈ

Clinical Significance of HBV DNA Detection in HBV M-Positive Sera Using Polymerase Chain Reaction

HBV M-positive sera from 140 patients with chronic hepatosis had been searched for HBV DNA using polymerase chain reaction (PCR). The results showed that 72. 86% (102/140)of the samples were positive for HBV DNA ;the HBV DNA frequency in HBsAg-positive. HBeAg-positive or anti-HBc-positive sera were 76. 56%(98/128) ,96. 15% (50/52) ,and 74. 42%(96/129) respectively. Of 12 HBsAg-negative sera, 4 were positive for HBV DNA (33. 3%); of 88 HBeAg-negative. sera, 52 were positive for HBV DNA(59. 09%). Of all the eight HBV infection patterns,the one which was positive for HBsAg.HBeAg and anti-HBc had the highest HBV DNA positivity of 96. 15% (50/52), significantly higher than other patterns(P<0. 025-0. 001). The results suggested that the antigenic-ity of HBsAg was not correlated to infectiousness;HBeAg was an important marker showing activity and infectiousness of HBV;anti-HBc,as a HBV marker,had its clinical special significance in regions with higher hepatocarcinoma risk. It confirmed that PCR amplification was a valuable tool for diagnosing HBV infection states,especially for observing curative effect or recurrence of hepatitis.