| Effects of lactulose on intestinal endotoxin and bacterial translocation in cirrhotic rats |
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| 作者姓名： | 张顺财 王唯 任卫英 戴茜 贺伯明 周康 |
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| 作者单位： | [1]DepartmentofGastroenterology,ZhongshanHospital,ShanghaiMedicalUniversity,Shanghai200032,China [2]InstituteofMateriaMedica,ChineseAcademyofScience,Shanghai200032,China [3]DepartmentofGastroenterology,ZhongshanHospital,Shanghai200032,China |
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| 基金项目： | hisstudywaspartiallysupportedbytheNationalNaturalScienceFundationofChina (No .30 0 70 340 ) |
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| 摘 要： | Objective To investigate the effects of lactulose on intestinal bacterial overgrowth (IBO), bacterial translocation (BT), intestinal transit and permeability in cirrhotic rats.Methods BT in all animals was assessed by bacterial culture of mesenteric lymph node ( MLN), liver and spleen, and IBO was assessed by a jejunal bacterial count of the specific organism. Intestinal permeability was determined by the 24-hour urinary ^99mTc-diethylenetriamine pentaacetatic acid (^99mTc-DTPA) excretion, and intestinal transit was determined by measuring the distribution of ^51Cr in the intestine.Results BT and IBO were found in 48% and 80% of the cirrhotic rats, respectively, while not in the control rats. Cirrhotic rats with IBO had significantly higher levels of intestinal endotoxin higher rates of bacterial translocation, shorter intestinal transit time and higher intestinal permeability than those without IBO. It was also found that BT were closely associated with IBO and injury of the intestinal barrier. Compared with the placebo group, lactulose-treated rats had lower rates of BT and IBO,which were closely associated with increased intestinal transit and improved intestinal permeability by lactulose.Conclusions Our study indicate that endotoxin and bacterial translocation in cirrhotic rats may attribute to IBO and increased intestinal permeability. Lactulose that accelerates intestinal transit and improves intestinal permeability might be helpful in preventing intestinal bacterial and endotoxin translocation.
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| 关 键 词： | 半乳糖苷果糖 肠内毒 细菌迁移 肝硬化 动物实验 |
Effects of lactulose on intestinal endotoxin and bacterial translocation in cirrhotic rats |
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| ZHANG Shuncai,WANG Wei,REN Weiying,DAI Qian,HE Boming and ZHOU Kang.Effects of lactulose on intestinal endotoxin and bacterial translocation in cirrhotic rats[J].Chinese Medical Journal,2003,116(5):767-771. |
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| Authors: | ZHANG Shuncai WANG Wei REN Weiying DAI Qian HE Boming and ZHOU Kang |
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| Institution: | 1. Department of Gastroenterology, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
2. Institute of Materia Medica, Chinese Academy of Science, Shanghai 200032, China |
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| Abstract: | Objective To investigate the effects of lactulose on intestinal bacterial overgrowth (IBO), bacterial translocation (BT), intestinal transit and permeability in cirrhotic rats. Methods BT in all animals was assessed by bacterial culture of mesenteric lymph node (MLN), liver and spleen, and IBO was assessed by a jejunal bacterial count of the specific organism. Intestinal permeability was determined by the 24-hour urinary 99mTc-diethylenetriamine pentaacetatic acid ( 99mTc-DTPA) excretion, and intestinal transit was determined by measuring the distribution of 51Cr in the intestine. Results BT and IBO were found in 48% and 80% of the cirrhotic rats, respectively, while not in the control rats. Cirrhotic rats with IBO had significantly higher levels of intestinal endotoxin higher rates of bacterial translocation, shorter intestinal transit time and higher intestinal permeability than those without IBO. It was also found that BT were closely associated with IBO and injury of the intestinal barrier. Compared with the placebo group, lactulose-treated rats had lower rates of BT and IBO, which were closely associated with increased intestinal transit and improved intestinal permeability by lactulose. Conclusions Our study indicate that endotoxin and bacterial translocation in cirrhotic rats may attribute to IBO and increased intestinal permeability. Lactulose that accelerates intestinal transit and improves intestinal permeability might be helpful in preventing intestinal bacterial and endotoxin translocation. |
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| Keywords: | bacterial translocation cirrhosis intestinal permeability lactulose endotoxin |
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