A Novel Reassortant H3N8 Influenza Virus Isolated from Drinking Water for Duck in a Domestic Duck Farm in Poyang Lake Area |
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| Authors: | DONG Bei Bei XU Cui Ling DONG Li Bo CHENG Hui Jian YANG Lei ZOU Shu Mei CHEN Min |
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| Affiliation: | DONG Bei Bei (Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention,China CDC, Beijing 102206, China; School of Public Health, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China)； XU Cui Ling (Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention,China CDC, Beijing 102206, China)； DONG Li Bo (Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention,China CDC, Beijing 102206, China)； CHENG Hui Jian (Jiangxi Province Center for Disease Control and Prevention, Jiangxi 330046, China)； YANG Lei (Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention,China CDC, Beijing 102206, China)； ZOU Shu Mei (Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention,China CDC, Beijing 102206, China)； CHEN Min (Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention,China CDC, Beijing 102206, China)； |
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| Abstract: | ObjectiveTo conduct a full genome sequence analysis for genetic characterization of an H3N8 influenza virus isolated from drinking water of a domestic duck farm in Poyang Lake area in 2011.MethodsThe virus was cultivated by specific pathogen free (SPF) chicken embryo eggs and was subtyped into hemagglutinin (HA) and neuraminidase (NA) by real-time PCR method. Eight gene segments were sequenced and phylogenetic analysis was conducted.ResultsThe NA gene of this virus belongs to North American lineage; other seven genes belong to Eurasian lineage. Compared with the viruses containing NA gene, the PB2 and PB1 gene came from different clades. And this indicates that the virus was a novel reassortant genotype. The HA receptor binding preference was avian-like and the cleavage site sequence showed a low pathogenic feature. There was no drug resistance mutation of M2 protein. The mutations of Asn30Asp, and Thr215Ala of the M1 protein implied the potential of pathogenicity increase in mice.ConclusionThe finding of novel genotype of H3N8 virus in drinking water in this duck farm near Poyang Lake highlighted the importance of strengthening the surveillance of avian influenza in this region, which could contribute to pinpointing the influenza ecological relations among avian, swine, and human. |
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| Keywords: | Avian influenza H3N8 Sequence analysis |
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