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| 中药虎杖中抗癌活性物质研究 | |
| 引用本文： | 冯磊,张莲芬,严婷,金坚,陶文沂. 中药虎杖中抗癌活性物质研究[J]. 中药材, 2006, 29(7): 689-691 |
| 作者姓名： | 冯磊 张莲芬 严婷 金坚 陶文沂 |
| 作者单位： | 1. 江南大学生物工程学院生物制药实验室,江苏,无锡,214036 2. 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 3. 江南大学生物工程学院生物制药实验室,江苏,无锡,214036;江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 |
| 摘 要： | 目的:为虎杖药用价值的开发利用提供科学据依。方法:在生物活性测定指导下,从虎杖中分离得到一种较高抗癌活性的物质,并且用液质联用、红外光谱、紫外全扫描等方法鉴定其结构。MTT法测定它对L-02、HepG2、SHZ-888、MCF-7、MCF-7/ADM等细胞的抑制作用并用HE染色法观察其作用MCF-7/ADM细胞48 h、72 h后的肿瘤细胞形态学变化。结果:分离得到的物质为顺、反式-白藜芦醇,它能特异性抑制多种肿瘤细胞的生长,而对正常肝细胞毒性很小。同时,首次发现其对耐阿霉素的MCF-7乳腺癌细胞有直接的细胞毒性。结论:白藜芦醇是虎杖中一种活性强、低毒的抗癌物质。 |
| 关 键 词： | 虎杖 白藜芦醇 MCF-7/ADM细胞 MTT法 |
| 文章编号： | 1001-4454(2006)07-0689-03 |
| 收稿时间： | 2005-10-31 |
| 修稿时间： | 2005-10-31 |
Studies on Active Substance of Anticancer Effect in Polygonum cuspidatum |
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| FENG Lei,ZHANG Lian-fen,YAN Ting,JIN Jian,TAO Wen-yi. Studies on Active Substance of Anticancer Effect in Polygonum cuspidatum[J]. Journal of Chinese medicinal materials, 2006, 29(7): 689-691 | |
| Authors: | FENG Lei ZHANG Lian-fen YAN Ting JIN Jian TAO Wen-yi |
| Affiliation: | 1. The Biopharmaceutical Laboratory, School of Biotechnology, Yangtze University,Wuxi 216036, China; 2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Yangtze University, Wuxi 214036, China |
| Abstract: | Objective: To supply the scientific basis of research and development of the medicinal value of Polygonum cuspidatum.Methods: One composition was isolated from the roots of Polygonum cuspidatum by cytotoxicity based fractionation and identified by HPLC-MS,UV scanning and IR.The inhibition and morphology of L-02,Hep G2,SHZ-888,MCF-7,MCF-7/ADM cells growth caused by this composition was determined by MTT assay and HE dyeing.Results: This composition was identified as trans-and cis-resveratrol.It could specifically inhibit proliferation of many cancer cells but not human normal liver cell.We investigated the cytotoxicity of resveratrol to adriamycin-resistant MCF-7 cell in virtro.Conclusion: Resveratrol is a new anticancer composition which is less toxicity and higher efficiency in Polygonum cuspidatum. |
| Keywords: | Polygonum cuspidatum Resveratrol MCF-7/ADM cell MTT assay |
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