锌对前列腺癌PC-3M细胞侵袭能力的影响

目的探讨锌对人前列腺癌PC-3M细胞侵袭能力的影响。方法用MIT比色法检测不同剂量锌对PC-3M细胞增殖的影响；Tramwell小室检测锌对PC-3M细胞侵袭能力的影响；RT-PCR检测锌作用24h后MMP-2、MMP-9mRNA的表达；酶谱分析检测锌作用后PC-3M细胞MMP-2、MMP-9的活性变化。结果MTT检测结果表明一定剂量的锌可抑制PC-3M细胞的生长；Transwell小室实验结果显示，锌作用下PC-3M细胞穿透Matrigel膜的细胞数减少；RT-PCR结果显示锌抑制了PC-3M细胞bIMP-2和MMP-9的表达；酶谱分析表明锌作用后PC-3M细胞MMP-2、MMP-9的活性下降。结论一定剂量的锌可降低PC-3M细胞的侵袭能力，其机制可能与抑制MMP-2、MMP-9的合成和激活有关。

The effect of zinc on the invasion ability in human prostate carcinoma cell PC-3M

Objective To investigate the effects of zinc on the mRNA expression of MMP-2 and MMP-9 of human prostate carcinoma cell PC-3M. Methods The effects of zinc with different concentractions on proliferation of PC-3M cell were detemained by MTT assay. Transwell chamber was used to investigate the effects of zinc on invasion abilities of PC-3M cell. The mRNA expression of MMP- 2 and MMP-9 were detected by RT-PCR synthesis after 24h in culture with zinc. The activation of MMP-2 and MMP-9 was detected by gelatin zynography. Results MTr assay showed that zinc with a certain concertration could inhibit proliferation of PC-3M cell. Under the effect of zinc,the number of PC-3M cell which moved through the matrigel membrane decrease.The result of RT-PCR and gelatin zynography respectively showed the synthesis and activation of MMP-2 and MMP-9 decreased after treated with zine.d. Conclusion Zinc with a certain concentraction can inhibit the proliferation and invasion abilities of PG-3M cell,and the mechanism is possibly relevant to the inhibitation of the synthesis and activation of MMP-2 and MMP-9.