聚己内酯/纳米羟基磷灰石静电纺纤维膜的制备及其生物活性

目的 检测静电纺丝技术制备的聚己内酯/纳米羟基磷灰石复合支架材料的理化表征、生物学性能及成骨活性。方法 通过静电纺丝技术制备聚己内酯/纳米羟基磷灰石复合材料,测量其理化表征。以MC3T3-E1细胞为模型初步评估复合材料的细胞相容性与成骨活性,为骨组织再生提供实验依据。结果 聚己内酯/纳米羟基磷灰石静电纺丝是一种无序三维交错网状结构,具有较小的拉伸强度及良好的润湿性。在聚己内酯/纳米羟基磷灰石复合纤维材料表面MC3T3-E1细胞生长良好,有伪足伸出,促进碱性磷酸酶及Runx2基因的表达。结论 聚己内酯/纳米羟基磷灰石静电纺纤维膜具有良好的理化表征、生物学性能与成骨特性,是一种潜在可用于种植骨再生的材料。

Preparation and bioactivity of polycaprolactone/nano hydroxyapatite electrospun fiber membrane

Objective To study the physical-chemical characteristics, biological properties and osteogenic activity of the polycaprolactone/nano-hydroxyapatite composite materials prepared by electrospinning technology.Methods The electrospinning technology was used to fabricate the polycaprolactone/nano-hydroxyapatite composite material, and the physical and chemical characterization were measured. And then MC3T3-E1 cells were used as the model to evaluate the biocompatibility and osteogenic activity of the composite material in order to provide experimental basis for its application in guided bone regeneration.Results The polycaprolactone/nano-hydroxyapatite composite material with a disordered non-woven three-dimensional staggered network structure can show low tensile strength and good wettability. MC3T3-E1 cells with pseudopodia can grow well on the polycaprolactone/nano hydroxyapatite membrane, which could promote the expression of alkaline phosphatase and Runx2 gene.Conclusion The polycaprolactone/nano hydroxyapatite electrospun membrane could show good physical-chemical characteristics, biological properties and osteogenic properties, which was a potential material for bone regeneration.