电针对心肌缺血再灌注损伤家兔心肌细胞凋亡的影响

目的:研究电针对心肌缺血再灌注损伤家兔心肌细胞凋亡的影响.方法:将结扎左冠状动脉前降支30 min,再灌注60 min,成功制作为心肌缺血再灌注损伤模型的家兔24只,随机分为模型组、电针1及2组各8只.另仅开胸未结扎冠状动脉的8只为假手术组作为正常对照.电针1、2组术后30 min分别采用电针针刺内关穴与列缺穴60 min,仅1次,应用原位末端标记法观察4组家兔心肌细胞凋亡情况.结果:心肌凋亡细胞数量,模型组显著高于假手术组(P＜0.01),电针1组与电针2组、模型组比较显著降低(P＜0.05,P＜0.01),但仍高于假手术组(P＜0.01).结论:细胞凋亡参与心肌缺血再灌注损伤的病理过程,电针内关穴可以减少缺血再灌注损伤心肌细胞凋亡的数量,从而达到抗缺血再灌注损伤的作用.

Effect of Electroacupuncture against Apoptosis of Myocardial Ischemia Reperfusion Injury in Rabbits

Objective: To study the effect of electroacupuncture(EA) against apoptosis of myocardial ischemia reperfusion injury in rabbits.Methods: Rabbits were divided into 4 groups: sham operating group,model group,EA at Neiguan points group,EA at Lieque points group.Myocardial ischemia reperfusion injury model was produced by ligating anterior descending branch of left coronary artery for 30 min and reperfusion for 60 min in rabbits.The effect of electroacupuncture(EA) against apoptosis of myocardial ischemia reperfusion injury in rabbits was observed.Results: The apoptotic number of myocardial in model group was significantly more than in sham operating group(P<0.01). Compared with model group and EA at Lieque points group,the apoptotic number of myocardial cells was significantly increased in EA at Neiguan points group.Conclution: Apoptosis involves in the pathological process of myocardial ischemia reperfusion in rabbits.EA at Neiguan point can decrease the apoptotic number of myocardial cells.