Studies on arsenictrioxide induced human pulmonary adenocarcinoma GLC-82 cell apoptosis and its molecularmechanisms |
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| Authors: | DENG You ping LIN Chen ZHANG Xue yan et al. |
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| Affiliation: | DENG You ping,LIN Chen,ZHANG Xue yan,et al.National Laboratory of Molecular Oncology,Cancer Institute,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100021,P.R. China |
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| Abstract: | Objective:To study the biological effect of As2O3 on human pulmonary adenocarcinoma GLC 82 cells and its mechanisms.Methods:MTT reduction assay,morphology investigation, flowcytometric analysis, DNA gel electrophoresis and TUNEL,RT PCR, Northern blot, Western blot methods were perfomed. Results:As2O3 inhibited the growth of GLC 82 cell line. The cells treated with As2O3 showed a typical apoptotic morphology and hypodiploid peak before G1 phase.DNA of treated GLC 82 cells appeared a ladder pattern characteristic of apoptosis.In situ cell death detection analysis also revealed the DNA fragmentation. Besides, As2O3 inhibited c myc gene expression and increased p53 and p16 gene expression. Conclusion:As2O3 can induce GLC 82 cell apoptosis mainly with regulation of c myc,p53 and p16 gene expression. |
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| Keywords: | Arsenic trioxide Human pulmonary adenocarcinoma GLC 82 cells apoptosis Gene expression |
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