吗啡预处理兔心肌MALDI-TOF-MS分析

目的：对吗啡预处理兔心肌进行基质辅助激光解吸飞行时间质谱（matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS）分析。方法：将6只新西兰大白兔随机分为对照组和吗啡预处理组，每组3只。对照组静脉注射生理盐水1 mL/kg, 吗啡预处理组静脉注射吗啡3 mg/kg。用双向凝胶电泳法分离两组24 h后的心肌组织蛋白，图像分析后找出差异蛋白。用MALDI-TOF-MS鉴定部分差异蛋白。结果：两组的差异明显点共有51个，取15个差异蛋白质进行质谱分析，初步鉴定出8个蛋白质，包括应激蛋白（醛糖还原酶、锌指蛋白312）、信号转导蛋白（Src相关的酪氨酸激酶）、代谢相关蛋白（碳酸酐酶12前体、电子转移黄素蛋白β亚单位、甘油醛-3-磷酸脱氢酶）、炎性反应相关蛋白（肿瘤坏死因子配体超家族成员11）和跨膜转运蛋白。结论：这些差异表达的蛋白可能在吗啡对心肌延迟性保护中发挥作用。

Proteomic analysis of morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

ObjectiveTo analyze the morphine rabbit myocardium with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry(MALDI-TOF-MS).MethodsSix New Zealand white rabbits were randomly assigned to a control group (Group C) and a morphine group(Group M). Group C were pretreated with bolus injection of saline 1mL/kg.Group M were pretreated with bolus injection of morphine 3 mg/kg. The myocardium tissue proteins of the rabbits 24 hours after the injection of morphine or saline preconditioned were extracted and separated by two dimensional gel electrophoresis(2-DE), and the images were analyzed and different proteins were found. Some of the different proteins were determined with MALDI-TOF-MS.ResultsThere were 51 protein spots that displaied quantitive changes in expression(P＜0.05）, 15 protein spots were choosen for MS analysis, and 8 proteins were preliminarily identified.They were aldose reductase,zinc finger protein 312,src related tyrosine kinase, carbonic anhydrase 12 precursor,electron transfer flavoprotein beta-subunit,glyceraldehyde-3-phosphate dehydrogenase, tumor necrosis factor ligand superfamily member 11 and transmembrane emp24 domain-containing protein. ConclusionThese proteins may be involved in the cardioprotection of morphine preconditioning.