Isolation and structure characterization of related impurities in 10-O-(N,N-dimethylaminoethyl)-ginkgolide B methanesulfonate (XQ-1H) bulk drug and quantitation by a validated RP-LC |
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Authors: | Wenyuan Liu Ping Li Feng Feng Lishun Mao |
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Affiliation: | 1. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;2. Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China;3. Department of Phytochemistry, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, Jiangsu, China;4. Jiangsu Carefree Medicinal Company Limited, Nanjing 210016, China |
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Abstract: | 10-O-(N,N-dimethylaminoethyl)-ginkgolide B methanesulfonate (XQ-1H), a novel active derivative of ginkgolide B, is a platelet-activating factor antagonist which is being under clinical trial. Two unknown related impurities were observed in analysis of XQ-1H bulk drug. A scaling up preparative liquid chromatography (Prep LC) was used for isolation of the two impurities. Based on LC–MS/MS and nuclear magnetic resonance (NMR) spectra, they were characterized as 10-O-(N,N-dimethylaminoethyl)-11,12-seco-ginkgolide B (imp-1) and 10-O-(N,N-dimethylaminoethyl)-11,12,2,15-diseco-3,14-dehydroginkgolide B (imp-2), respectively. A reversed-phase liquid chromatography (RP-LC) was developed for simultaneous determination of XQ-1H as well as imp-1 and imp-2. Main variables that significantly influence the chromatographic procedure were optimized and efficient chromatographic separation was achieved on a CN column with mobile phase consisting of 5 mM dipotassium hydrogen phosphate (pH 7.5) and methanol delivered in a gradient mode at the flow rate of 1.0 mL min−1. The method was validated and found to be suitable to check the quality of bulk samples of XQ-1H at test concentration of 5.0 mg mL−1 for a 20 μL injection volume. |
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Keywords: | 10-O-(N,N-dimethylaminoethyl)-ginkgolide B methanesulfonate Related impurities Preparative liquid chromatography Reversed-phase liquid chromatography |
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